Caveolin 1 antibody

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Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with caveolin 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to caveolin 1 (molecular weight 21 kDa)

Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of Caveolin 1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti Caveolin 1 antibody (**VPA00698**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 426 second and (B) 60 second exposure.

Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of Caveolin 1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti Caveolin 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00698 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human caveolin 1 antibody recognizes caveolin-1 also known as cell growth-inhibiting protein 32, CAV1, or PPH3 .

The scaffolding protein encoded by CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene CAV1 is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in CAV1 have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1 (provided by RefSeq, Mar 2010).

Rabbit anti Human caveolin 1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Rabbit anti caveolin 1 detects a band of approximately 21 kDa in A549 cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human caveolin 1
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q03135
Entrez Gene: CAV1
GO Terms: GO:0000139 Golgi membrane; GO:0000188 inactivation of MAPK activity; GO:0005515 protein binding; GO:0001570 vasculogenesis; GO:0001666 response to hypoxia; GO:0001937 negative regulation of endothelial cell proliferation; GO:0007596 blood coagulation; GO:0005102 receptor binding; GO:0005783 endoplasmic reticulum; GO:0005811 lipid particle; GO:0005901 caveola; GO:0006641 triglyceride metabolic process; GO:0006816 calcium ion transport; GO:0006940 regulation of smooth muscle contraction; GO:0007519 skeletal muscle tissue development; GO:0009267 cellular response to starvation; GO:0010524 positive regulation of calcium ion transport into cytosol; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0015485 cholesterol binding; GO:0016050 vesicle organization; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0016504 peptidase activator activity; GO:0019217 regulation of fatty acid metabolic process; GO:0019915 lipid storage; GO:0030193 regulation of blood coagulation; GO:0030514 negative regulation of BMP signaling pathway; GO:0030857 negative regulation of epithelial cell differentiation; GO:0031295 T cell costimulation; GO:0031623 receptor internalization; GO:0032091 negative regulation of protein binding; GO:0032507 maintenance of protein location in cell; GO:0032570 response to progesterone stimulus; GO:0032947 protein complex scaffold; GO:0033137 negative regulation of peptidyl-serine phosphorylation; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0033484 nitric oxide homeostasis; GO:0042632 cholesterol homeostasis; GO:0051260 protein homooligomerization; GO:0043409 negative regulation of MAPKKK cascade; GO:0043627 response to estrogen stimulus; GO:0044419 interspecies interaction between organisms; GO:0045019 negative regulation of nitric oxide biosynthetic process; GO:0045907 positive regulation of vasoconstriction; GO:0046209 nitric oxide metabolic process; GO:0048471 perinuclear region of cytoplasm; GO:0048554 positive regulation of metalloenzyme activity; GO:0050900 leukocyte migration; GO:0050998 nitric-oxide synthase binding; GO:0050999 regulation of nitric-oxide synthase activity; GO:0051480 cytosolic calcium ion homeostasis; GO:0051592 response to calcium ion; GO:0051899 membrane depolarization; GO:0060056 mammary gland involution; GO:0070836 caveola assembly; GO:0090090 negative regulation of canonical Wnt receptor signaling pathway; GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Caveolin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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