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Caveolin 1 antibody

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Western Blot Validated
Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with caveolin 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to caveolin 1 (molecular weight 21 kDa)
Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of Caveolin 1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti Caveolin 1 antibody (VPA00698) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 426 second and (B) 60 second exposure.
Anti Caveolin 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of Caveolin 1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti Caveolin 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00698
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human caveolin 1 antibody recognizes caveolin-1 also known as cell growth-inhibiting protein 32, CAV1, or PPH3 .

The scaffolding protein encoded by CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene CAV1 is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in CAV1 have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1 (provided by RefSeq, Mar 2010).

Rabbit anti Human caveolin 1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti caveolin 1 detects a band of approximately 21 kDa in A549 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human caveolin 1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q03135
Entrez Gene
CAV1
GO Terms
GO:0000139 Golgi membrane
GO:0000188 inactivation of MAPK activity
GO:0005515 protein binding
GO:0001570 vasculogenesis
GO:0001666 response to hypoxia
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007596 blood coagulation
GO:0005102 receptor binding
GO:0005783 endoplasmic reticulum
GO:0005811 lipid particle
GO:0005901 caveola
GO:0006641 triglyceride metabolic process
GO:0006816 calcium ion transport
GO:0006940 regulation of smooth muscle contraction
GO:0007519 skeletal muscle tissue development
GO:0009267 cellular response to starvation
GO:0010524 positive regulation of calcium ion transport into cytosol
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015485 cholesterol binding
GO:0016050 vesicle organization
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016504 peptidase activator activity
GO:0019217 regulation of fatty acid metabolic process
GO:0019915 lipid storage
GO:0030193 regulation of blood coagulation
GO:0030514 negative regulation of BMP signaling pathway
GO:0030857 negative regulation of epithelial cell differentiation
GO:0031295 T cell costimulation
GO:0031623 receptor internalization
GO:0032091 negative regulation of protein binding
GO:0032507 maintenance of protein location in cell
GO:0032570 response to progesterone stimulus
GO:0032947 protein complex scaffold
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033484 nitric oxide homeostasis
GO:0042632 cholesterol homeostasis
GO:0051260 protein homooligomerization
GO:0043409 negative regulation of MAPKKK cascade
GO:0043627 response to estrogen stimulus
GO:0044419 interspecies interaction between organisms
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045907 positive regulation of vasoconstriction
GO:0046209 nitric oxide metabolic process
GO:0048471 perinuclear region of cytoplasm
GO:0048554 positive regulation of metalloenzyme activity
GO:0050900 leukocyte migration
GO:0050998 nitric-oxide synthase binding
GO:0050999 regulation of nitric-oxide synthase activity
GO:0051480 cytosolic calcium ion homeostasis
GO:0051592 response to calcium ion
GO:0051899 membrane depolarization
GO:0060056 mammary gland involution
GO:0070836 caveola assembly
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Caveolin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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