Caveolin 1 antibody

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Rabbit anti Caveolin 1

Caveolin 1 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00698 WB 100 µl
VPA00698KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human caveolin 1 antibody recognizes caveolin-1 also known as cell growth-inhibiting protein 32, CAV1, or PPH3 .

The scaffolding protein encoded by CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene CAV1 is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in CAV1 have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1 (provided by RefSeq, Mar 2010).

Rabbit anti Human caveolin 1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti caveolin 1 detects a band of approximately 21 kDa in A549 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human caveolin 1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q03135 Related reagents
Entrez Gene
CAV1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0000188 inactivation of MAPK activity
GO:0005515 protein binding
GO:0001570 vasculogenesis
GO:0001666 response to hypoxia
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007596 blood coagulation
GO:0005102 receptor binding
GO:0005783 endoplasmic reticulum
GO:0005811 lipid particle
GO:0005901 caveola
GO:0006641 triglyceride metabolic process
GO:0006816 calcium ion transport
GO:0006940 regulation of smooth muscle contraction
GO:0007519 skeletal muscle tissue development
GO:0009267 cellular response to starvation
GO:0010524 positive regulation of calcium ion transport into cytosol
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015485 cholesterol binding
GO:0016050 vesicle organization
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016504 peptidase activator activity
GO:0019217 regulation of fatty acid metabolic process
GO:0019915 lipid storage
GO:0030193 regulation of blood coagulation
GO:0030514 negative regulation of BMP signaling pathway
GO:0030857 negative regulation of epithelial cell differentiation
GO:0031295 T cell costimulation
GO:0031623 receptor internalization
GO:0032091 negative regulation of protein binding
GO:0032507 maintenance of protein location in cell
GO:0032570 response to progesterone stimulus
GO:0032947 protein complex scaffold
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033484 nitric oxide homeostasis
GO:0042632 cholesterol homeostasis
GO:0051260 protein homooligomerization
GO:0043409 negative regulation of MAPKKK cascade
GO:0043627 response to estrogen stimulus
GO:0044419 interspecies interaction between organisms
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045907 positive regulation of vasoconstriction
GO:0046209 nitric oxide metabolic process
GO:0048471 perinuclear region of cytoplasm
GO:0048554 positive regulation of metalloenzyme activity
GO:0050900 leukocyte migration
GO:0050998 nitric-oxide synthase binding
GO:0050999 regulation of nitric-oxide synthase activity
GO:0051480 cytosolic calcium ion homeostasis
GO:0051592 response to calcium ion
GO:0051899 membrane depolarization
GO:0060056 mammary gland involution
GO:0070836 caveola assembly
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Caveolin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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