Calpain-5 antibody

Rabbit anti Calpain-5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Calpain-5

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VPA00746
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Rabbit anti Human calpain-5 antibody recognizes calpain-5, also known as calpain htra-3 or new calpain 3.

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3 (provided by RefSeq, Jul 2008).

Rabbit anti Human calpain-5 antibody detects a band of 73 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti calpain-5 antibody recognizes a band of approximately 73 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Immunogen
Recombinant protein encompassing part of the center region of human calpain-5
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O15484
Entrez Gene
CAPN5
GO Terms
GO:0005622 intracellular
GO:0004198 calcium-dependent cysteine-type endopeptidase activity
GO:0007165 signal transduction
GO:0006508 proteolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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