BDNF antibody
Rabbit anti Human BDNF antibody (VPA00760) used to evaluate active BDNF (mBDNF) lacking the propeptide region, in murine hippocampal lysates by western blotting.
Image caption:
hippocampal samples of cbg+/+ and cbg-/- mice: mBDNF protein levels. Western blot shows 4 representative samples for each genotype.
From: Gulfo J, Pérez de San Román J, Ledda A, Junyent F, Ramírez MJ, Gil-Bea FJ, et al. (2021)
Corticosteroid-binding-globulin (CBG)-deficient mice show high pY216-GSK3β and phosphorylated-Tau levels in the hippocampus.
PLoS ONE 16(2): e0246930.
doi. 10.1371/journal.pone.0246930.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human BDNF antibody recognizes brain-derived neutotrophic factor (BDNF), also known as ANON2, BULN2, abrinurion and neurotrophin.
This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders (provided by RefSeq, Nov 2015).
Rabbit anti Human BDNF antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders (provided by RefSeq, Nov 2015).
Rabbit anti Human BDNF antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti BDNF antibody recognizes a band of approximately 28 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Mammals Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to a portion of the middle region of human BDNF
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P23560
- Entrez Gene
- BDNF
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of BDNF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
Product Specific References
References for BDNF antibody
-
Gulfo, J. et al. (2021) Corticosteroid-binding-globulin (CBG)-deficient mice show high pY216-GSK3β and phosphorylated-Tau levels in the hippocampus.
PLoS One. 16 (2): e0246930.
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