Bcl-2 antibody

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Anti Bcl-2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Bcl-2 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to Bcl-2 (molecular weight 26 kDa).

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Rabbit anti Bcl-2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Bcl-2

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VPA00775 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human Bcl-2 antibody recognizes Bcl2, also known as apoptosis regulator Bcl-2.

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants (provided by RefSeq, Feb 2016).

Rabbit anti Human Bcl-2 detects at a band of 26 kDa.

Target Species: Human
Western Blotting: Anti Bcl-2 antibody recognizes a band of approximately 26 kDa in THP-1 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. col-derived recombinant protein of amino acids 1-211 of human Bcl-2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P10415
Entrez Gene: BCL2
GO Terms: GO:0000209 protein polyubiquitination; GO:0001836 release of cytochrome c from mitochondria; GO:0002020 protease binding; GO:0016021 integral to membrane; GO:0005741 mitochondrial outer membrane; GO:0005789 endoplasmic reticulum membrane; GO:0006916 anti-apoptosis; GO:0006959 humoral immune response; GO:0006974 response to DNA damage stimulus; View More GO Terms; GO:0007565 female pregnancy; GO:0008629 induction of apoptosis by intracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0042493 response to drug; GO:0009636 response to toxin; GO:0010039 response to iron ion; GO:0016563 transcription activator activity; GO:0030307 positive regulation of cell growth; GO:0030890 positive regulation of B cell proliferation; GO:0031965 nuclear membrane; GO:0032848 negative regulation of cellular pH reduction; GO:0034097 response to cytokine stimulus; GO:0035094 response to nicotine; GO:0042100 B cell proliferation; GO:0042803 protein homodimerization activity; GO:0043496 regulation of protein homodimerization activity; GO:0043497 regulation of protein heterodimerization activity; GO:0043524 negative regulation of neuron apoptosis; GO:0043565 sequence-specific DNA binding; GO:0046902 regulation of mitochondrial membrane permeability; GO:0046982 protein heterodimerization activity; GO:0050853 B cell receptor signaling pathway; GO:0051402 neuron apoptosis; GO:0051434 BH3 domain binding; GO:0051607 defense response to virus; GO:0051881 regulation of mitochondrial membrane potential; GO:0051902 negative regulation of mitochondrial depolarization; GO:0070059 apoptosis in response to endoplasmic reticulum stress