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Aurora-B Kinase antibody

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Anti Aurora-B Kinase Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Aurora-B Kinase Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with aurora-B kinase antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 139 second exposure. Arrow points to aurora-B kinase (molecular weight 48 kDa).

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Rabbit anti Aurora-B Kinase

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Aurora-B Kinase

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Rabbit anti Human aurora-B kinase antibody recognizes aurora B-kinase, also known as ARK-2, STK-1, aurora 1, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora-related kinase 2, aurora/IPL1-related kinase 2, serine/threonine-protein kinase 12 or serine/threonine-protein kinase B.

Encoded by the AURKB gene, aurora-B kinase is a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for AURKB (provided by RefSeq, Jan 2013).

Rabbit anti Human aurora-B kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 283-313 of human aurora-B kinase
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
Q96GD4
Entrez Gene
AURKB
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000780 condensed nuclear chromosome, centromeric region
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005819 spindle
GO:0006468 protein phosphorylation
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0032133 chromosome passenger complex
GO:0034501 protein localization to kinetochore
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