Aurora-B Kinase antibody

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Rabbit anti Aurora-B Kinase

Rabbit anti-human Aurora-B kinase antibody recognizes Aurora kinase B, also known as ARK-2, STK-1, aurora 1, aurora- and Ipl1-like midbody-associated protein 1, protein phosphatase 1, regulatory subunit 48, or serine/threonine kinase 12.

Aurora-B Kinase Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00485	WB	100 µl
VPA00485KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human aurora-B kinase antibody recognizes aurora B-kinase, also known as ARK-2, STK-1, aurora 1, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora-related kinase 2, aurora/IPL1-related kinase 2, serine/threonine-protein kinase 12 or serine/threonine-protein kinase B.

Encoded by the AURKB gene, aurora-B kinase is a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for AURKB (provided by RefSeq, Jan 2013).

Rabbit anti Human aurora-B kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid.

Product Form

Purified IgG - liquid.

Preparation

Rabbit Ig fraction prepared by ammonium sulphate precipitation.

Preparation

20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation.

Buffer Solution

Phosphate buffered saline.

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃).

Preservative Stabilisers

0.09% Sodium Azide (NaN₃).

Immunogen

KLH conjugated synthetic peptide corresponding to amino acid 283-313 of human aurora-B kinase

Instructions For Use

Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q96GD4 Related reagents
Entrez Gene: AURKB Related reagents
GO Terms: GO:0005515 protein binding; GO:0000236 mitotic prometaphase; GO:0000780 condensed nuclear chromosome, centromeric region; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005819 spindle; GO:0006468 protein phosphorylation; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0032133 chromosome passenger complex; GO:0034501 protein localization to kinetochore
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only.

Applications of Aurora-B Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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