Aurora-B Kinase antibody

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Rabbit anti Aurora-B Kinase

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00485 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human aurora-B kinase antibody recognizes aurora B-kinase, also known as ARK-2, STK-1, aurora 1, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora-related kinase 2, aurora/IPL1-related kinase 2, serine/threonine-protein kinase 12 or serine/threonine-protein kinase B.

Encoded by the AURKB gene, aurora-B kinase is a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for AURKB (provided by RefSeq, Jan 2013).

Rabbit anti Human aurora-B kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid.

Preparation

Rabbit Ig fraction prepared by ammonium sulphate precipitation.

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃).

Immunogen

KLH conjugated synthetic peptide corresponding to amino acid 283-313 of human aurora-B kinase

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q96GD4
Entrez Gene: AURKB
GO Terms: GO:0005515 protein binding; GO:0000236 mitotic prometaphase; GO:0000780 condensed nuclear chromosome, centromeric region; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005819 spindle; GO:0006468 protein phosphorylation; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0032133 chromosome passenger complex; GO:0034501 protein localization to kinetochore
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only.

Applications of Aurora-B Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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