Aurora-B Kinase antibody

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Rabbit anti Aurora-B Kinase

Rabbit anti-human Aurora-B kinase antibody recognizes Aurora kinase B, also known as ARK-2, STK-1, aurora 1, aurora- and Ipl1-like midbody-associated protein 1, protein phosphatase 1, regulatory subunit 48, or serine/threonine kinase 12.

Aurora-B Kinase Antibody with Control Lysate

Rabbit anti-human Aurora-B kinase antibody recognizes Aurora kinase B, also known as ARK-2, STK-1, aurora 1, aurora- and Ipl1-like midbody-associated protein 1, protein phosphatase 1, regulatory subunit 48, or serine/threonine kinase 12.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00485 WB 100 µl
VPA00485KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human aurora-B kinase antibody recognizes aurora B-kinase, also known as ARK-2, STK-1, aurora 1, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora-related kinase 2, aurora/IPL1-related kinase 2, serine/threonine-protein kinase 12 or serine/threonine-protein kinase B.

Encoded by the AURKB gene, aurora-B kinase is a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for AURKB (provided by RefSeq, Jan 2013).

Rabbit anti Human aurora-B kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Product Form
Purified IgG - liquid.
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 283-313 of human aurora-B kinase
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q96GD4 Related reagents
Entrez Gene
AURKB Related reagents
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000780 condensed nuclear chromosome, centromeric region
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005819 spindle
GO:0006468 protein phosphorylation
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0032133 chromosome passenger complex
GO:0034501 protein localization to kinetochore
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of Aurora-B Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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