ATF3 antibody
Rabbit anti Human ATF3 antibody recognizes cyclic AMP-dependent transcription factor ATF3 or cyclic AMP-dependent transcription factor ATF-3.
The ATF3 gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. ATF3 is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for ATF3. It is possible that alternative splicing of ATF3 may be physiologically important in the regulation of target genes (provided by RefSeq, Apr 2011).
Rabbit anti Human ATF3 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The ATF3 gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. ATF3 is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for ATF3. It is possible that alternative splicing of ATF3 may be physiologically important in the regulation of target genes (provided by RefSeq, Apr 2011).
Rabbit anti Human ATF3 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide encompassing part of the middle region of human ATF3
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P18847 Related reagents
- Entrez Gene
- ATF3 Related reagents
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0005730 nucleolus
- GO:0042802 identical protein binding
- GO:0043565 sequence-specific DNA binding
- GO:0046983 protein dimerization activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ATF3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Rabbit anti ATF3 detects a band of approximately 21 kDa in Jurkat cell lysates
- Western Blotting
- Rabbit anti ATF3 detects a band of approximately 21 kDa in Jurkat cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
