ASS1 antibody

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Goat anti Human ASS1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1669 E IP P* WB 0.1 mg
Goat anti human ASS1 antibody recognises human argininosuccinate synthetase-1, an ornithine (urea) cycle enzyme which catalyzes the penultimate step of the arginine biosynthetic pathway.

Defects in ASS1 genes are responsible for the autosomal recessive disease known as citrullinemia type 1 (CTLN1), characterized by increased levels of urine and serum citrulline, and ammonia intoxication.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Dog Expected from Sequence
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ASS1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-ENPKNQAPPGLYTKTQD from the internal region of ASS1 (NP_000041.2; NP_446464.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00966 Related reagents
Entrez Gene
ASS1 Related reagents
GO Terms
GO:0000050 urea cycle
GO:0005515 protein binding
GO:0004055 argininosuccinate synthase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006526 arginine biosynthetic process
Regulatory
For research purposes only

Applications of ASS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/64000
Immunohistology - Paraffin 1 2.0 4.0ug/ml
Immunoprecipitation
Western Blotting 0.01 0.03ug/ml
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrated buffer, pH6.0, is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney
Western Blotting
Goat anti human ASS1 detects a band of approximately 45kDa in human kidney cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P