ASS1 antibody

Goat anti Human ASS1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ASS1

Product Code Applications Pack Size List Price Your Price Qty
AHP1669
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP P * WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti human ASS1 antibody recognises human argininosuccinate synthetase-1, an ornithine (urea) cycle enzyme which catalyzes the penultimate step of the arginine biosynthetic pathway.

Defects in ASS1 genes are responsible for the autosomal recessive disease known as citrullinemia type 1 (CTLN1), characterized by increased levels of urine and serum citrulline, and ammonia intoxication.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Dog Expected from Sequence
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ASS1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-ENPKNQAPPGLYTKTQD from the internal region of ASS1 (NP_000041.2; NP_446464.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/64000
Immunohistology - Paraffin 1 2.0 4.0ug/ml
Immunoprecipitation
Western Blotting 0.01 0.03ug/ml
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrated buffer, pH6.0, is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney
Western Blotting
Goat anti human ASS1 detects a band of approximately 45kDa in human kidney cell lysates

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

Synonyms
CTLN1
RRID
AB_2060468
UniProt
P00966
Entrez Gene
ASS1
GO Terms
GO:0000050 urea cycle
GO:0005515 protein binding
GO:0004055 argininosuccinate synthase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006526 arginine biosynthetic process
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products