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ARF6 antibody

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Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2
Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ARF6 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 27 second exposure. Arrow points to ARF6 (molecular weight 19 kDa)
Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Immunoprecipitation of ARF6 from C6 whole cell lysate. The Input lane is 10% input; the ARF6-IP lane is the immunoprecipitation with the ARF6 antibody (2.5 µg, VPA00668); the IgG-IP lane is the immunoprecipitation with Rabbit polyclonal IgG (2.5 µg). Western blot analysis was performed using ARF6 antibody (1/1,000, VPA00668) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, 1705046) and visualized on the ChemiDoc MP. Arrow points to ARF6.

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WB IP

Rabbit anti ARF6

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
ARF6

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VPA00668
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Rabbit anti Human ARF6 antibody recognizes ADP-ribosylation factor 6

The ARF6 gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of ARF6 is located on chromosome 7 (provided by RefSeq, Jul 2008).

Rabbit anti Human ARF6 antibody detects a band of 19 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti ARF6 detects a band of approximately 19 kDa in C6 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human ARF6
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P62330
Entrez Gene
ARF6
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0001726 ruffle
GO:0016192 vesicle-mediated transport
GO:0007155 cell adhesion
GO:0005525 GTP binding
GO:0005624 membrane fraction
GO:0005794 Golgi apparatus
GO:0005938 cell cortex
GO:0006928 cellular component movement
GO:0007264 small GTPase mediated signal transduction
GO:0010008 endosome membrane
GO:0015031 protein transport
GO:0030838 positive regulation of actin filament polymerization
GO:0030866 cortical actin cytoskeleton organization
GO:0031529 ruffle organization
GO:0031996 thioesterase binding
GO:0035020 regulation of Rac protein signal transduction
GO:0048261 negative regulation of receptor-mediated endocytosis
GO:0060998 regulation of dendritic spine development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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