ARF6 antibody

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Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ARF6 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 27 second exposure. Arrow points to ARF6 (molecular weight 19 kDa)

Anti ARF6 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Immunoprecipitation of ARF6 from C6 whole cell lysate. The Input lane is 10% input; the ARF6-IP lane is the immunoprecipitation with the ARF6 antibody (2.5 µg, **VPA00668**); the IgG-IP lane is the immunoprecipitation with Rabbit polyclonal IgG (2.5 µg). Western blot analysis was performed using ARF6 antibody (1/1,000, **VPA00668**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, 1705046) and visualized on the ChemiDoc MP. Arrow points to ARF6.

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WB IP

Rabbit anti ARF6

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ARF6

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00668 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Rabbit anti Human ARF6 antibody recognizes ADP-ribosylation factor 6

The ARF6 gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of ARF6 is located on chromosome 7 (provided by RefSeq, Jul 2008).

Rabbit anti Human ARF6 antibody detects a band of 19 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti ARF6 detects a band of approximately 19 kDa in C6 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide encompassing part of the middle region of human ARF6
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P62330
Entrez Gene: ARF6
GO Terms: GO:0005886 plasma membrane; GO:0003924 GTPase activity; GO:0001726 ruffle; GO:0016192 vesicle-mediated transport; GO:0007155 cell adhesion; GO:0005525 GTP binding; GO:0005624 membrane fraction; GO:0005794 Golgi apparatus; GO:0005938 cell cortex; View More GO Terms; GO:0006928 cellular component movement; GO:0007264 small GTPase mediated signal transduction; GO:0010008 endosome membrane; GO:0015031 protein transport; GO:0030838 positive regulation of actin filament polymerization; GO:0030866 cortical actin cytoskeleton organization; GO:0031529 ruffle organization; GO:0031996 thioesterase binding; GO:0035020 regulation of Rac protein signal transduction; GO:0048261 negative regulation of receptor-mediated endocytosis; GO:0060998 regulation of dendritic spine development