Apolipoprotein L1 antibody

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Anti Human Apolipoprotein L1 (C-Terminal) Antibody thumbnail image 1

Anti Human Apolipoprotein L1 (C-Terminal) Antibody thumbnail image 2

Anti Human Apolipoprotein L1 (C-Terminal) Antibody gallery image 1 — Western blotting analysis of human placental lysate (35μg protein in RIPA buffer) probed with Goat anti Human Apolipoprotein L1 antibody (**AHP1896**) at 0.1μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human Apolipoprotein L1 (C-Terminal) Antibody gallery image 2 — Western blot analysis of human frontal cortex lysate probed with Goat anti human apolipoprotein L1 antibody (**AHP1896**).

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Goat anti Human Apolipoprotein L1 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Apolipoprotein L1
Region: (C-TERMINAL)

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AHP1896 Datasheet Datasheet SDS SDS	E WB	0.1 mg		Log in	Get Quote
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Goat anti Human apolipoprotein L1 antibody recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma.

Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver.

Goat anti Human apolipoprotein L1 is expected to recognise both reported isoforms of human APOL1.

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human APOL1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Synthetic peptide C-NNNYKILQADQE from within the C-Terminal region of human APOL1 (NP_003652.2; NP_663318.1).
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/8000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1896 detects a band of approximately 45kDa in human brain (frontal cortex) cell lysates

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RRID: AB_2058404
UniProt: O14791
Entrez Gene: APOL1
GO Terms: GO:0005515 protein binding; GO:0005254 chloride channel activity; GO:0008289 lipid binding; GO:0006869 lipid transport; GO:0008203 cholesterol metabolic process; GO:0019835 cytolysis; GO:0031224 intrinsic to membrane; GO:0031640 killing of cells of other organism; GO:0034361 very-low-density lipoprotein particle; View More GO Terms; GO:0034364 high-density lipoprotein particle; GO:0042157 lipoprotein metabolic process; GO:0045087 innate immune response