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Apolipoprotein L1 antibody

Goat anti Human Apolipoprotein L1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Apolipoprotein L1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1896
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader

Goat anti Human apolipoprotein L1 antibody recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma.

Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver.

Goat anti Human apolipoprotein L1 is expected to recognise both reported isoforms of human APOL1.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human APOL1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide C-NNNYKILQADQE from within the C-Terminal region of human APOL1 (NP_003652.2; NP_663318.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1896 detects a band of approximately 45kDa in human brain (frontal cortex) cell lysates

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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Description Rabbit anti Goat IgG (Fc):HRP

RRID
AB_2058404
UniProt
O14791
Entrez Gene
APOL1
GO Terms
GO:0005515 protein binding
GO:0005254 chloride channel activity
GO:0008289 lipid binding
GO:0006869 lipid transport
GO:0008203 cholesterol metabolic process
GO:0019835 cytolysis
GO:0031224 intrinsic to membrane
GO:0031640 killing of cells of other organism
GO:0034361 very-low-density lipoprotein particle
GO:0034364 high-density lipoprotein particle
GO:0042157 lipoprotein metabolic process
GO:0045087 innate immune response

AHP1896

160511

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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