Apolipoprotein B antibody

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Goat anti Human Apolipoprotein B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1267
Datasheet
E IP WB
SDS
0.1 mg

Goat anti Human Apolipoprotein B antibody detects human Apolipoprotein B (ApoB), a large secretory protein with a molecular weight of ~550 kDa that plays a central role in lipoprotein metabolism.

ApoB exists in the plasma in 2 main isoforms, ApoB100 and ApoB48. ApoB100 is synthesised in the liver, and is the major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL), and is absolutely required for their formation.ApoB100 acts as a ligand for LDL receptors in various cells throughout the body, allowing cells to internalise LDL and absorb cholesterol.

ApoB48 is expressed in the intestine and is present on chylomicrons and their remnants, and lacks the C-terminal LDL-receptor binding region of ApoB100. Elevated plasma concentrations of ApoB containing lipoproteins are key risk factors for the development of atherosclerosis.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Apolipoprotein B were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
125mM Sodium Borate
75mM Sodium Chloride
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
5mM EDTA
Immunogen
Apolipoprotein type B, isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P04114
Entrez Gene
APOB
Regulatory
For research purposes only

Applications of Apolipoprotein B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/10,000
Immunoprecipitation
Western Blotting 1/200 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1267 detects a band of approximately 550kDa.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for Apolipoprotein B antibody

  1. Philippova, M. et al. (2019) Analysis of fragmented oxidized phosphatidylcholines in human plasma using mass spectrometry: Comparison with immune assays.
    Free Radic Biol Med. 144: 167-75.

Further Reading

  1. Olofsson, S.O. & Borèn, J. (2005) Apolipoprotein B: a clinically important apolipoprotein which assembles atherogenic lipoproteins and promotes the development of atherosclerosis.
    J Intern Med. 258 (5): 395-410.
  2. Walldius, G. & Jungner, I. (2004) Apolipoprotein B and apolipoprotein A-I: risk indicators of coronary heart disease and targets for lipid-modifying therapy.
    J Intern Med. 255 (2): 188-205.

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