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Annexin II antibody

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Anti Human Annexin II (N-Terminal) Antibody thumbnail image 1
Anti Human Annexin II (N-Terminal) Antibody gallery image 1
Western blotting of A431 lysate (35μg total protein in RIPA buffer) probed with Goat anti Human annexin II antibody (OBT1388) at 0.5μgml-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence.

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Goat anti Human Annexin II (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Annexin II
Region
(N-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
OBT1388
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader

Goat anti Human Annexin II (N-Terminal) antibody recognizes an epitope within the N terminal (NT) region of Annexin II, a member of a family of structurally related calcium binding proteins implicated in mediating the the intracellular action of calcium. Cellular levels of annexin II mRNA and protein are strictly regulated during the mammalian cell cycle with maximal expression as cells enter S-phase.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse
Bovine Expected from Sequence
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Annexin II was raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence STVHEILCKLSLEGD corresponding to the N-terminal region of Annexin II (NP_001002858.1; NP_004030.1; NP_001002857.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000
Western Blotting 1 ug/ml 3 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
OBT1388 detects a band of approximately 35 kDa in A431 and A549 cell lysates

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References for Annexin II antibody

  1. Xie, R. et al. (2012) Phagocytosis by macrophages and endothelial cells inhibits procoagulant and fibrinolytic activity of acute promyelocytic leukemia cells.
    Blood. 119 (10): 2325-34.

RRID
AB_2057321
UniProt
P07355
Entrez Gene
ANXA2
GO Terms
GO:0001501 skeletal system development
GO:0005509 calcium ion binding
GO:0004859 phospholipase inhibitor activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005604 basement membrane
GO:0005625 soluble fraction
GO:0008092 cytoskeletal protein binding
GO:0030496 midbody
GO:0031340 positive regulation of vesicle fusion
GO:0042470 melanosome

OBT1388

160726

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