AIF1 antibody

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Anti AIF1 (C-Terminal) Antibody thumbnail image 1

Anti AIF1 (C-Terminal) Antibody thumbnail image 2

Anti AIF1 (C-Terminal) Antibody thumbnail image 3

Anti AIF1 (C-Terminal) Antibody thumbnail image 4

Anti AIF1 (C-Terminal) Antibody thumbnail image 5

Anti AIF1 (C-Terminal) Antibody gallery image 1 — Immunohistochemical staining of formalin fixed, paraffin embedded mouse brain with Goat anti Human AIF1 antibody (**AHP2024**) following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0)

Anti AIF1 (C-Terminal) Antibody gallery image 2 — Western blotting of Human (A) Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer) probed with Goat anti Human AIF1 antibody (**AHP2024**) at 1.0μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti AIF1 (C-Terminal) Antibody gallery image 3 — Western blot analysis of rat brain lysate probed with Goat anti Human AIF1 antibody (**AHP2024**) followed by HRP conjugated anti Goat antibody and detection by chemiluminescence.

Anti AIF1 (C-Terminal) Antibody gallery image 4 — **Published customer image:**
Goat anti AIF1 antibody (**AHP2024**) used to label microglial cells in rat hippocampus by immunofluorescence.
**Image caption:**
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].

From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti AIF1 (C-Terminal) Antibody gallery image 5 — **Published customer image:**
Goat anti AIF1 antibody (**AHP2024**) used to label microglial cells in rat hippocampus by immunofluorescence.
**Image caption:**
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].

From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti AIF1 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP2024 Datasheet	E P* WB SDS	0.1 mg	Log in		Get Quote

Goat anti AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.

AIF1 was first identified in rat cardiac allografts with chronic rejection (Utans et al. 1995), is expressed by activated monocytes and macrophages (Orsmark et al. 2007) and plays a significant role in vascular inflammation (Chen et al. 2004)

Goat anti AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wang et al. 2014).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Pig	Expected from Sequence
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Immunogen

Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P55008; P55009
Entrez Gene: AIF1; Aif1
GO Terms: GO:0005509 calcium ion binding; GO:0051015 actin filament binding; GO:0051017 actin filament bundle assembly; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005856 cytoskeleton; GO:0006954 inflammatory response; GO:0007050 cell cycle arrest; GO:0008285 negative regulation of cell proliferation; GO:0032587 ruffle membrane
Regulatory: For research purposes only

Applications of AIF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/32000
Immunohistology - Paraffin ¹	2.0ug/ml
Western Blotting	1.0	3.0ug/ml

¹This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Tris/EDTA buffer pH9.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP2024 detects a band of approximately 16kDa in rat brain cell lysates.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Product Specific References

References for AIF1 antibody

Wang, X. et al. (2014) Pseudoginsenoside-F11 (PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways.
Neuropharmacology. 79: 642-56.
Kang, D.H. et al. (2020) Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 14: 568813.
Wu, C. et al. (2021) E3 ubiquitin ligase Triad1 promotes neuronal apoptosis by regulating the p53-caspase3 pathway after spinal cord injury.
Somatosens Mot Res. : 1-8.

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