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AIF1 antibody

Goat anti AIF1 (C-Terminal)

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
List Price Your Price

Goat anti AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.

AIF1 was first identified in rat cardiac allografts with chronic rejection (Utans et al. 1995), is expressed by activated monocytes and macrophages (Orsmark et al. 2007) and plays a significant role in vascular inflammation (Chen et al. 2004)

Goat anti AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wang et al. 2014).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 1.0 3.0ug/ml
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Tris/EDTA buffer pH9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2024 detects a band of approximately 16kDa in rat brain cell lysates.

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Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for AIF1 antibody

  1. Kang, D.H. et al. (2020) Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
    Front Neurosci. 14: 568813.
  2. Wu, C. et al. (2022) E3 ubiquitin ligase Triad1 promotes neuronal apoptosis by regulating the p53-caspase3 pathway after spinal cord injury.
    Somatosens Mot Res. 39 (1): 21-8.
  3. Zhou, C. et al. (2022) Mild Hypothermia Protects Brain Injury After Intracerebral Hemorrhage in Mice Via Enhancing the Nrdp1/MyD88 Signaling Pathway.
    Neurotox Res. 40 (6): 1664-72.
  4. Sheu, M.L. et al. (2023) Thrombin-Induced Microglia Activation Modulated through Aryl Hydrocarbon Receptors.
    Int J Mol Sci.24 (14):11416.
  5. Lu, X. et al. (2018) HAX1 is associated with neuronal apoptosis and astrocyte proliferation after spinal cord injury.
    Tissue Cell. 54: 1-9.

Entrez Gene
GO Terms
GO:0005509 calcium ion binding
GO:0051015 actin filament binding
GO:0051017 actin filament bundle assembly
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0006954 inflammatory response
GO:0007050 cell cycle arrest
GO:0008285 negative regulation of cell proliferation
GO:0032587 ruffle membrane


159698 170421

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