ABL2 antibody
Rabbit anti Human ABL2 antibody recognizes ABL proto-oncogene 2 also known as ARG.
This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. ABL2 is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. ARG is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene (provided by RefSeq, Nov 2009).
Rabbit anti Human ABL2 antibody detects a band of 128 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. ABL2 is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. ARG is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene (provided by RefSeq, Nov 2009).
Rabbit anti Human ABL2 antibody detects a band of 128 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti ABL2 detects a band of approximately 128 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered glycine
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Immunogen
- Recombinant protein encompassing part of the middle region of human ABL2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P42684 Related reagents
- Entrez Gene
- ABL2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000287 magnesium ion binding
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0007165 signal transduction
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0007411 axon guidance
- GO:0018108 peptidyl-tyrosine phosphorylation
- GO:0030145 manganese ion binding
- GO:0051353 positive regulation of oxidoreductase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ABL2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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