ABCB5 antibody

Goat anti Human ABCB5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ABCB5

Product Code Applications Pack Size List Price Your Price Qty
AHP1667
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human ABCB5 antibody recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine (Frank et al. 2003). ABCB5 is primarily expressed by skin progenitor cells and acts as a regulator of cell fusion and determinant of membrane potential. ABCB5 is expressed in human malignant melanoma, and is associated with CD133+ stem cell phenotype-expressing cells.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ABCB5 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QTQHRNTSKKAQ from the internal region of ABCB5 (NP_848654.3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Western Blotting 0.03 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1667 detects a band of approximately 80kDa in A431cell lysates

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for ABCB5 antibody

  1. Parmakhtiar, B. et al. (2019) HIF Inactivation of p53 in Ovarian Cancer Can Be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity.
    Mol Cancer Res. 17 (8): 1675-86.

Further Reading

  1. Moitra, K. et al. (2011) Molecular evolutionary analysis of ABCB5: the ancestral gene is a full transporter with potentially deleterious single nucleotide polymorphisms.
    PLoS One. 6: e16318.

RRID
AB_2220822
UniProt
Q2M3G0
Entrez Gene
ABCB5
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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