ABCB5 antibody
Goat anti Human ABCB5 antibody recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine. ABCB5 is primarily expressed by skin progenitor cells and shown to act as a regulator of cell fusion and determinant of membrane potential. Studies have revealed the expression of ABCB5 in human malignant melanoma tumours, and in particular its association with CD133+ stem cell phenotype-expressing cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ABCB5 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QTQHRNTSKKAQ from the internal region of ABCB5 (NP_848654.3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of ABCB5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/128000 | ||
| Western Blotting | 0.03 | 0.1ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1667 detects a band of approximately 80kDa in A431cell lysates
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for ABCB5 antibody
-
Parmakhtiar, B. et al. (2019) HIF Inactivation of p53 in Ovarian Cancer Can Be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity.
Mol Cancer Res. 17 (8): 1675-86.
Further Reading
-
Moitra, K. et al. (2011) Molecular evolutionary analysis of ABCB5: the ancestral gene is a full transporter with potentially deleterious single nucleotide polymorphisms.
PLoS One. 6: e16318.
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