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ABCB5 antibody

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Anti Human ABCB5 Antibody gallery image 1
Western blotting analysis of lysates of A431 cell line lysate (35μg protein in RIPA buffer) probed with Goat anti Human ABCB5 antibody (AHP1667) at 0.3μgml-1. Primary incubation was 1 hour. Detected by chemiluminescence

Goat anti Human ABCB5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1667
Datasheet
E WB
SDS
0.1 mg

Goat anti Human ABCB5 antibody recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine. ABCB5 is primarily expressed by skin progenitor cells and shown to act as a regulator of cell fusion and determinant of membrane potential. Studies have revealed the expression of ABCB5 in human malignant melanoma tumours, and in particular its association with CD133+ stem cell phenotype-expressing cells.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ABCB5 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QTQHRNTSKKAQ from the internal region of ABCB5 (NP_848654.3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q2M3G0
Entrez Gene
ABCB5
Regulatory
For research purposes only

Applications of ABCB5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Western Blotting 0.03 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1667 detects a band of approximately 80kDa in A431cell lysates
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for ABCB5 antibody

  1. Parmakhtiar, B. et al. (2019) HIF Inactivation of p53 in Ovarian Cancer Can Be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity.
    Mol Cancer Res. 17 (8): 1675-86.

Further Reading

  1. Moitra, K. et al. (2011) Molecular evolutionary analysis of ABCB5: the ancestral gene is a full transporter with potentially deleterious single nucleotide polymorphisms.
    PLoS One. 6: e16318.

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Request a different product with the ABCB5 specificity