A4GALT antibody

Rabbit anti A4GALT

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
A4GALT

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VPA00585
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Rabbit anti Human A4GALT antibody recognizes A4GALT, also known as CD77 synthase, GB3 synthase, P blood group (P one antigen), P(k) antigen synthase, P1/Pk synthase, UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase, alpha-1,4-N-acetylglucosaminyltransferase, alpha4Gal-T1 or globotriaosylceramide synthase.

The protein encoded by the A4GALT gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, a single pass, type II membrane glycoprotein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor (provided by RefSeq, Jul 2008).

Rabbit anti Human A4GALT antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti A4GALT detects a band of approximately 40 kDa in HT-1080 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human A4GALT
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q9NPC4
Entrez Gene
A4GALT
GO Terms
GO:0005624 membrane fraction
GO:0005795 Golgi stack
GO:0006688 glycosphingolipid biosynthetic process
GO:0007009 plasma membrane organization
GO:0030173 integral to Golgi membrane
GO:0050512 lactosylceramide 4-alpha-galactosyltransferase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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