A4GALT antibody

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Rabbit anti A4GALT

Rabbit anti-human A4GALT antibody recognizes lactosylceramide 4-alpha-galactosyltransferase, also known as CD77 synthase, GB3 synthase, P blood group (P one antigen), or UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00585 WB 100 µl
Rabbit anti Human A4GALT antibody recognizes A4GALT, also known as CD77 synthase, GB3 synthase, P blood group (P one antigen), P(k) antigen synthase, P1/Pk synthase, UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase, alpha-1,4-N-acetylglucosaminyltransferase, alpha4Gal-T1 or globotriaosylceramide synthase.

The protein encoded by the A4GALT gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, a single pass, type II membrane glycoprotein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor (provided by RefSeq, Jul 2008).

Rabbit anti Human A4GALT antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti A4GALT detects a band of approximately 40 kDa in HT-1080 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human A4GALT
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9NPC4 Related reagents
Entrez Gene
A4GALT Related reagents
GO Terms
GO:0005624 membrane fraction
GO:0005795 Golgi stack
GO:0006688 glycosphingolipid biosynthetic process
GO:0007009 plasma membrane organization
GO:0030173 integral to Golgi membrane
GO:0050512 lactosylceramide 4-alpha-galactosyltransferase activity
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of A4GALT antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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