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IgM antibody

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Goat anti Horse IgM:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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Goat anti Horse IgM polyclonal antibody recognizes equine IgM.

No cross-reactivity with other equine immunoglobulin classes is seen in immunoelectrophoresis. This antibody may cross-react with IgM from other species.

Target Species
Horse
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to equine IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified equine IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgM antibody

  1. Jacks, S. et al. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
    Clin Vaccine Immunol. 14: 669-77.
  2. Ryan, C. and Giguère, S. (2010) Equine Neonates Have Attenuated Humoral and Cell-Mediated Immune Responses to a Killed Adjuvanted Vaccine Compared to Adult Horses
    Clin Vaccine Immunol. 17: 1896-902.
  3. Swadzba, M.E. et al. (2012) Vitreal IgM Autoantibodies Target Neurofilament Medium in a Spontaneous Model of Autoimmune Uveitis.
    Invest Ophthalmol Vis Sci. 53: 294-300.
  4. Barrell, E.A. et al. (2017) Acute leukemia in six horses (1990-2012).
    J Vet Diagn Invest. 29 (4): 529-35.
  5. Arroyo, L.G. et al. (2022) Serum IgM antibody response to Clostridioides difficile polysaccharide PS-II vaccination in pony foals.
    Anaerobe. 77: 102635.

ELISA

RRID
AB_323034

AAI33F

161393 167622 180117

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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