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IgG antibody

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Anti Horse IgG (T) Antibody thumbnail image 1
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Published customer image:
Horseradish peroxidase conjugated Goat anti Horse IgGT antibody used for the determination of allergen specific antibody titre in horse serum by ELISA.
Image caption:
Healthy horses have allergen-specific serum antibodies. Allergen-specific antibody titers, for different isotypes, were determined in the serum of healthy (dark bars) and IBH-sensitive animals (dark bars) by ELISA. Bars represent average +SEM; n=6 and n=10, for healthy and IBH-sensitive animals, respectively; dots represent individual animals.

From: Meulenbroeks C, van der Lugt JJ, van der Meide NMA, Willemse T, Rutten VPMG, et al. (2015)
Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity.
PLoS ONE 10(4): e0122090.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Horse IgG (T):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(T)

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Goat anti Horse IgG (T) antibody recognizes equine IgG (T). No cross-reactivity with other equine immunoglobulin classes is seen in immuno-electrophoresis.

Goat anti Horse IgG (T) antibody may cross react with IgG from other species.

Target Species
Horse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to equine IgG (T) were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified equine IgG (T).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/20 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Hooper-McGrevy, K.E. et al. (2003) Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins.
    Clin Diagn Lab Immunol. 10 (3): 345-51.
  2. Jacks, S. et al. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
    Clin Vaccine Immunol. 14: 669-77.
  3. Lewis, M.J. et al. (2007) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
    Mol Immunol. 45: 818-27.
  4. Ryan, C. & Giguère, S. (2010) Equine neonates have attenuated humoral and cell-mediated immune responses to a killed adjuvanted vaccine compared to adult horses.
    Clin Vaccine Immunol. 17 (12): 1896-902.
  5. Cauchard S et al. (2014) Assessment of the safety and immunogenicity of Rhodococcus equi-secreted proteins combined with either a liquid nanoparticle (IMS 3012) or a polymeric (PET GEL A) water-based adjuvant in adult horses and foals--identification of promising new candidate antigens.
    Vet Immunol Immunopathol. 157 (3-4): 164-74.
  6. Meulenbroeks C et al. (2015) Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity.
    PLoS One. 10 (4): e0122090.
  7. Burk, S.V. et al. (2016) Equine antibody response to larval Parascaris equorum excretory-secretory products.
    Vet Parasitol. 226: 83-7.
  8. Lightbody, K.L. et al. (2016) Validation of a novel saliva-based ELISA test for diagnosing tapeworm burden in horses.
    Vet Clin Pathol. 45 (2): 335-46.
    9. View The Latest Product References
  9. Tzelos, T. et al. (2020) Characterisation of serum IgG(T) responses to potential diagnostic antigens for equine cyathostominosis.
    Int J Parasitol. 50 (4): 289-98.

ELISA

Western Blotting

RRID
AB_323022

AAI38F

148560 158085 162389

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