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IgA antibody

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Published customer image:
Horseradish peroxidase conjugated Goat anti Horse IgA antibody (AAI34P) used to measure antigen specific IgA levels in horse saliva samples by ELISA.
Image caption:
Results of the 3rd Stage of the study: Mean reactivity (SD) of antibodies in saliva in IgG, IgM and IgA classes in horses free from invasion and manifesting diverse intensity of invasion u with antigens L3 G. intestinalis in the ELISA test (A = 492 nm).

From: Pawlas-Opiela M, Jawor P, Galli J, Zak-Bochenek A, Gorczykowski M, Galli J, Sołtysiak Z, Stefaniak T.
The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.
Sci Rep. 2022 Oct 20;12(1):17573.
doi: 10.1038/s41598-022-21482-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Horse IgA:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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Product Code Applications Pack Size List Price Your Price Qty
AAI34P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader

Goat anti Horse IgA antibody recognizes equine IgA and shows no cross-reactivity with other equine immunoglobulin classes in immunoelectrophoresis. Goat anti Horse IgA antibody may cross-react with IgA from other species.

Target Species
Horse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to equine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Immunogen
Purified equine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgA antibody

  1. Lewis, M.J. et al. (2010) IgA in the horse: cloning of equine polymeric Ig receptor and J chain and characterization of recombinant forms of equine IgA.
    Mucosal Immunol. 3: 610-21.
  2. van der Kolk, J.H. et al. (2012) Gluten-dependent antibodies in horses with inflammatory small bowel disease (ISBD).
    Vet Q. 32: 3-11.
  3. Keggan, A. et al. (2013) Production of seven monoclonal equine immunoglobulins isotyped by multiplex analysis.
    Vet Immunol Immunopathol. 153 (3-4): 187-93.
  4. Palm, A.E. et al. (2016) Secretory immunoglobulin A and immunoglobulin G in horse saliva.
    Vet Immunol Immunopathol. 180: 59-65.
  5. Pawlas-Opiela, M. et al. (2022) The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.
    Sci Rep. 12 (1): 17573.
  6. May, A. et al. (2024) Effect of hospitalization on equine local intestinal immunoglobulin A (IgA) concentration measured in feces.
    J Equine Vet Sci. : 105078.

RRID
AB_323030

AAI34P

155237 155304 158972 160485 160903 166706

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