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IgM antibody

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Anti Bovine IgM Antibody thumbnail image 1
Anti Bovine IgM Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Sheep anti Bovine IgM antibody (AAI19P) used as a detection antibody in an ELISA to evaluate IgM responses to D.viviparus. infection.
Image caption:
Anti-rMSP antibody development during vaccination trials. Mean optical density (OD) values are displayed over the course of the study for immunoglobulin (Ig) classes IgG, IgA and IgM for the different study groups. Error bars indicate standard deviations. V = vaccination, C = challenge infection.

From: Springer, A.; von Holtum, C.; von Samson-Himmelstjerna, G.; Strube, C.
Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus.
Pathogens 2022, 11, 55.
doi: 10.3390/pathogens11010055
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Sheep anti Bovine IgM:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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Product Code Applications Pack Size List Price Your Price Qty
AAI19P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader

Sheep anti bovine IgM recognizes bovine IgM and shows no cross-reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. Sheep anti bovine IgM may cross-react with IgM from other species.

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Immunogen
Purified bovine IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgM antibody

  1. Nebl, T. et al. (2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
    J Biol Chem. 277 (45): 43399-409.
  2. Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
    Vet Immunol Immunopathol. 147: 25-34.
  3. Hamsten, C. et al. (2009) Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
    Mol Cell Proteomics. 8: 2544-54.
  4. Mansilla FC et al. (2015) Safety and immunogenicity of a soluble native Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle.
    Vet Immunol Immunopathol. 165 (1-2): 75-80.
  5. Hossain, M.M. et al. (2016) Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.
    Vector Borne Zoonotic Dis. 16 (8): 550-7.
  6. Van Meulder, F. et al. (2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora.
    Int J Parasitol. 45 (9-10): 637-46.
  7. Jankowska, A. et al. (2016) Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animals
    Veterinární Medicína. 60 (No. 11): 603-12.
  8. Di Giacomo, S et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle
    Viruses. 14 (8): 1781.
    9. View The Latest Product References
  9. Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 2022, 11, 55.

ELISA

Western Blotting

RRID
AB_323075

AAI19P

147084 155147 156865 164142

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