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IgA antibody

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Anti Bovine IgA Antibody thumbnail image 1
Anti Bovine IgA Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Sheep anti Bovine IgA antibody (AAI49P) used as a detection antibody in an ELISA to evaluate IgA responses to D.viviparus. infection.
Image caption:
Anti-rMSP antibody development during vaccination trials. Mean optical density (OD) values are displayed over the course of the study for immunoglobulin (Ig) classes IgG, IgA and IgM for the different study groups. Error bars indicate standard deviations. V = vaccination, C = challenge infection.

From: Springer, A.; von Holtum, C.; von Samson-Himmelstjerna, G.; Strube, C.
Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus.
Pathogens 2022, 11, 55.
doi: 10.3390/pathogens11010055
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Sheep anti Bovine IgA:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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Sheep anti Bovine IgA antibody recognizes bovine IgA and shows no cross reactivity with other bovine immunoglobulin classes in immunoelectrophoresis.This antibody may recognize IgA from other species, though this has not been verified.

IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhearing to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.

In addition to this polyclonal antibody, we have a monoclonal Mouse anti Bovine IgA , clone IL-A71 antibody (MCA2438GA ) available which is specific for bovine IgA, also available in a peroxidase conjugated format.

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to bovine IgA were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Immunogen
Purified bovine IgA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Immunocytochemistry 1/200 1/2000
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/2000 1/20,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgA antibody

  1. Calvo-Lorenzo, M.S. et al. (2016) Wooden hutch space allowance influences male Holstein calf health, performance, daily lying time, and respiratory immunity.
    J Dairy Sci. 99 (6): 4678-92.
  2. González-Hernández A et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
    Sci Rep. 6: 29496.
  3. Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 11, 55.

Further Reading

  1. Morton, H.C. et al. (2004) Cloning and characterization of an immunoglobulin A Fc receptor from cattle.
    Immunology. 111 (2): 204-11.

AAI49P

153983 159836 160901 163932

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