pNPP

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  • pNPP
  • pNPP
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF044AEdatasheet pdfdatasheet pdf100 ml
    BUF044A
    BUF044BEdatasheet pdfdatasheet pdf500 ml
    BUF044B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Intended Use
      Pack Size: 500 mlBUF044B is a high performance p-nitrophenol phosphate (pNPP) solution, recommended for use in ELISA as a substrate for alkaline phosphatase (AP). In the presence of AP, pNPP is hydolyzed rapidly to p-nitrophenol and inorganic phosphate to produce a yellow solution.

      This ready to use solution has been carefully formulated to increase pNPP activity and stability. BUF044B may be used for both kinetic and endpoint tests.
      Pack Size: 100 mlBUF044A is a high performance p-nitrophenol phosphate (pNPP) solution, recommended for use in ELISA as a substrate for alkaline phosphatase (AP). In the presence of AP, pNPP is hydolyzed rapidly to p-nitrophenol and inorganic phosphate to produce a yellow solution.

      This ready to use solution has been carefully formulated to increase pNPP activity and stability. BUF044A may be used for both kinetic and endpoint tests.
      Pack Size: 500 mlBUF044B is a high performance p-nitrophenol phosphate (pNPP) solution, recommended for use in ELISA as a substrate for alkaline phosphatase (AP). In the presence of AP, pNPP is hydolyzed rapidly to p-nitrophenol and inorganic phosphate to produce a yellow solution.

      This ready to use solution has been carefully formulated to increase pNPP activity and stability. BUF044B may be used for both kinetic and endpoint tests.
      Pack Size: 100 mlBUF044A is a high performance p-nitrophenol phosphate (pNPP) solution, recommended for use in ELISA as a substrate for alkaline phosphatase (AP). In the presence of AP, pNPP is hydolyzed rapidly to p-nitrophenol and inorganic phosphate to produce a yellow solution.

      This ready to use solution has been carefully formulated to increase pNPP activity and stability. BUF044A may be used for both kinetic and endpoint tests.
    • Product Form
      Ready to use - liquid
      Ready to use - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present.
      None present.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product is photosensitive and should be protected from light.

      Avoid exposure to light and heat

      Store aliquots in bottles made of High Density Polyethylene (HDPE).
      Store at +4oC. DO NOT FREEZE.
      This product is photosensitive and should be protected from light.

      Avoid exposure to light and heat

      Store aliquots in bottles made of High Density Polyethylene (HDPE).
    • Shelf Life
      12 months from date of despatch.
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. Remove the pNPP substrate container from the refrigerator, and allow it to reach room temperature prior to use.

      2. Add 100ul of BUF044B per microtiter well. Development time is typically 15-30 minutes. Optimum time is determined by the user and will vary according to the procedure and the incubation temperature.

      3. For kinetic tests, measure absorbance at 405nm. For endpoint tests, add 100ul 1.0M sodium hydroxide solution to each well and mix throughly prior to measuring absorbance at 405nm /650nm.
    • Instructions For Use
      1. Remove the pNPP substrate container from the refrigerator, and allow it to reach room temperature prior to use.

      2. Add 100ul of BUF044A per microtiter well. Development time is typically 15-30 minutes. Optimum time is determined by the user and will vary according to the procedure and the incubation temperature.

      3. For kinetic tests, measure absorbance at 405nm. For endpoint tests, add 100ul 1.0M sodium hydroxide solution to each well and mix throughly prior to measuring absorbance at 405nm /650nm.

    Additional pNPP Formats

    Formats Applications Sizes available
    pNPP : Ready To Use E 100 ml | 500 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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