Browsing Modes for Business Users

MCP-1

Product Code Applications Pack Size List Price Your Price Qty
PPP016
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 5 µg loader
List Price Your Price
loader

Recombinant Pig MCP-1 is an 8.6 kDa peptide corresponding to aa 24 – 99 of porcine monocyte chemotactic protein 1 (MCP-1). Also known as CCL2 , MCP-1 is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
8.6 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant porcine MCP-1 may be used as a standard in a sandwich ELISA assay in conjunction with AHP2384 as a coating antibody and AHP2384B as a detection antibody.

References for MCP-1

  1. Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
    Res Vet Sci. 131: 266-300.

Synonyms
CCL2
UniProt
P42831
Entrez Gene
CCL2
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity

PPP016

136889 163809 167660

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with MCP-1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Pig Products
Please note