IFN Gamma

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Pig Interferon Gamma ELISA

Recombinant Pig Interferon gamma (PPP022) detected using Rabbit anti Pig interferon gamma (AHP2390) as the capture reagent and Rabbit anti Pig Interferon gamma:Biotin (AHP2390B) as the detection reagent followed by Streptavidin:HRP

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  • Recombinant Pig Interferon Gamma
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PPP022E, WBdatasheet pdfdatasheet pdf5 µg
    PPP022
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant Pig interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

      Native pig interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.

      IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      16.8 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at -20oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      interferon-gamma receptor binding
      immune response
      cytokine activity
      response to virus
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2390 as a coating antibody and AHP2390B as a detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Rec. Protein E, WB 5 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti Pig Interferon GammaHCA0900.1 mgE
          HCA090

          Recommended Positive Controls

            Histology Controls

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