Endothelial Cells Antibody | MIL11

//www.bio-rad-antibodies.com/pig-porcine-endothelial-cells-antibody-mil11-mca1752.html?RenderPageSpecific=1
Endothelial Cells Antibody | MIL11 gallery image 1

Published customer image:
Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of porcine jujenal vasculature by immunofluorescence.
Image caption:
Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 µm. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 μm. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p<0.01. doi:10.1371/journal.pone.0033707.g001

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012) Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.

Enlarge
Endothelial Cells Antibody | MIL11 gallery image 2

Published customer image:
Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of porcine jujenal vasculature by immunofluorescence.
Image caption:
Colonisation expands mucosal CD4+ T cells by 21 days of age. (A) CD4 staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to CD4 (green) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the blue channel (endothelium); (iii) shows the original greyscale image for the green channel (CD4). Scale bar represents 10 μm. (C) Area of CD4+ T cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). *p<0.05.

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012) Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.

Enlarge
Endothelial Cells Antibody | MIL11 gallery image 3

Published customer image:
Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of endothelium in porcine melanoma by immunofluorescence.
Image caption:
Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.

From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 2015;48(1):15-26.

Enlarge
Endothelial Cells Antibody | MIL11 gallery image 4

Published customer image:
Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of endothelium in porcine melanoma by immunofluorescence.
Image caption:
Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.

From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 2015;48(1):15-26.

Enlarge
  • Endothelial Cells Antibody | MIL11 thumbnail image 1
  • Endothelial Cells Antibody | MIL11 thumbnail image 2
  • Endothelial Cells Antibody | MIL11 thumbnail image 3
  • Endothelial Cells Antibody | MIL11 thumbnail image 4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MIL11
  • Isotype
    IgE
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1752Cdatasheet pdfdatasheet pdf2 ml
    MCA1752
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig endothelial cells, clone MIL11 raised initially against an immunogen of plastic adherent lymphocytes has been shown to recognize a major subset of porcine endothelial cells. MIL11 was initially identified as staining a characteristic pattern of capillary endothelium in gut forming a network in the lamina propria below the epithelial membrane. Staining was subsequently observed in capillary endothelium in most organs examined, including gut, lung, kidney and skin. Clone MIL11 was observed not to stain endothelium of aorta and other arteries whereas venous endothelium was positive for MIL11 staining. Some population of venous endothelial cells however appear negative for MIL11 staining including those associated with the heart wall, kidney glomeruli and corpus cavernosum (Wilson et al.1996) .
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Plastic adherent porcine peripheral blood lymphocytes.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/201/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Endothelial Cells Antibody Formats

    Formats Clone Applications Sizes available
    Endothelial Cells Antibody : S/N MIL11 C 2 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

              Write your review

              You may also be interested in...