CD45RC Antibody | MIL15

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CD45RC Antibody | MIL15 gallery image 1

Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgM isotype control (MCA692) detected with goat anti mouse IgM PE (STAR86PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM (STAR86PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD45RC Antibody | MIL15 gallery image 2

Figure A. Purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM PE (STAR86PE) and mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM PE (STAR86PE) and mouse anti porcine CD3 FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD45RC
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  • Product Type
    Monoclonal Antibody
  • Clone
    MIL15
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1750C, F, Pdatasheet pdfdatasheet pdf2 ml
    MCA1750
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman, F. A. et al. 2001).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Cells isolated from porcine mesenteric lymph node.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45RC Antibody Formats

    Formats Clone Applications Sizes available
    CD45RC Antibody : S/N MIL15 C, F, P 2 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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