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Multiple IgG1/IgG2a/IgG1 Negative Control Antibody

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  • Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control
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  • Product Type
    Negative/Isotype Control
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC023F *datasheet pdfdatasheet pdf100 Tests/1ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Multiple IgG1/IgG2a/IgG1 negative control is a suitable isotype control for the measurement of non-specific binding of mouse IgG1, rat IgG2a and rat IgG1 monoclonal antibodies, conjugated to FITC, RPE and Alexa Fluor ® 647 respectively, in three colour flow cytometry experiments.

      This product is appropriate for use in experiments targeting human, canine and porcine cells.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized.
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      FITC reagent: IgG1 (MOUSE)
      RPE reagent: IgG2a (RAT)
      A647 reagent: IgG1 (RAT)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)
      It is recomended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Multiple IgG1/IgG2a/IgG1 Negative Control Antibody Formats

    Formats Applications Sizes available
    Multiple IgG1/IgG2a/IgG1 Negative Control Antibody : 3 Color F * 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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