Mouse Serum

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Mouse serum (C11SA) used as a component of the wash buffer prior to incubation with anti mouse secondary antibody to reduce any non specific binsing of the anti mouse immunoglobulin during flow cytometric analysis of cell surface primary mouse antibody binding.
Image caption:
β cells are antigenically heterogeneous in normal and pathological islets.
Human islet samples were enzymatically dispersed and antibody labelled for flow cytometric analysis, and β cells were isolated by FACS with the sorting scheme illustrated in . (a,b) Representative examples of CD9 versus ST8SIA1 expression on the purified β cell populations from two healthy islet donors. (c–j) CD9 versus ST8SIA1 expression on cells from eight islet samples collected from T2D patients. (k) Mean β cell subtype distribution (±s.d.) from 17 normal islet preps. Significant frequency variance (P<1 × 10-24, single factor analysis of variance) was observed between these populations and β1 is significantly more frequent (P=8 × 10-4, 1.2 × 10-12, 5 × 10-13 by t-test (unequal variance)) than any of the others and β2 was significantly more frequent than β3 or β4 (1 × 10-8, 2 × 10-9 by t-test (unequal variance)) as indicated with asterisks. (l) The mean percentage (±s.d.) of ST8SIA1+ β cells is abnormally high (P=0.028, t-test (unequal variance)) in T2D islets (n=8) compared with normal islets (n=17).

From: Dorrell C, Schug J, Canaday PS, Russ HA, Tarlow BD, Grompe MT, Horton T, Hebrok M, Streeter PR, Kaestner KH, Grompe M.
Human islets contain four distinct subtypes of β cells.
Nat Commun. 2016 Jul 11;7:11756.

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  • Mouse Serum
  • Mouse Serum:Preservative Free
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  • Product Type
    Serum & Cells
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    C11SDC, IY, Pdatasheet pdfdatasheet pdf100 ml
    C11SD
    C11SDZC, IY, Pdatasheet pdfdatasheet pdf100 ml
    C11SDZ
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
      Whole serum - liquid
      Whole serum - liquid
    • Reconstitution
    • Preparation
      Normal mouse serum, 0.45um filtered. Prepared from barrier mice which are screened for infectious agents.
      Normal mouse serum, 0.2um filtered. Prepared from barrier mice which are screened for infectious agents.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      None present
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Mouse Serum Formats

    Formats Applications Sizes available
    Mouse Serum : Preservative Free C, IY, P 100 ml
    Mouse Serum : Serum C, IY, P 100 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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