Mouse IgM Negative Control Antibody

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Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgM isotype control (MCA692) detected with goat anti mouse IgM PE (STAR86PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM (STAR86PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse IgM Negative Control:FITC
  • Mouse IgM Negative Control
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    Mouse IgM isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgM
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA692FFdatasheet pdfdatasheet pdf0.1 mg
      MCA692F
      MCA692Fdatasheet pdfdatasheet pdf100 Tests
      MCA692
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Mouse IgM negative control antibody is designed for use as a negative control reagent to assess the level of non-specific binding of mouse IgM monoclonal antibodies to human cells.

        This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgM - liquid
      • Reconstitution
      • Preparation
        Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
        Purified IgM prepared by ammonium sulphate precipiation from tissue culture supernatant.
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
      • Purity
      • Approx. Protein Concentrations
        IgM concentration 0.1 mg/ml
        IgM concentration 0.1mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
      • Storage
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood.
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Mouse IgM Negative Control Antibody Formats

      Formats Applications Sizes available
      Mouse IgM Negative Control Antibody : FITC F 0.1 mg
      Mouse IgM Negative Control Antibody : Purified F 100 Tests
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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