IgG

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Purified Mouse IgG used for injection into rats to monitor antibody deposition in pripheral nerve tissues by immunofluorescence
Image caption:
Antibodies accumulate in peripheral nerves after passive transfer and lead to macrophage increase. (A) Immunohistochemical localization of antibodies after systemic injection of IgG in the femoral quadriceps (q) and saphenous nerve (s) from 6-month-old P0wt JHD-/- (7 days post injection, DPI) and P0het JHD-/- mice (7DPI and 28DPI). Note stronger IgG immunoreactivity in the endoneurium from P0het JHD-/- mice that received consecutive IgG injections over 28 days (28DPI). Scale bar, 20 µm. (B) Quantification of F4/80-positive profiles in non-injected control and reconstituted mice showing an increase of macrophage numbers in femoral quadriceps nerve from P0het JHD-/- mutants after IgG reconstitution (n?=?3 to 5). One-way ANOVA with Bonferroni-Holm correction. *P?
From: Klein, D., Groh, J., Weishaupt, A., & Martini, R. (2015). Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B.
Journal of Neuroinflammation, 12, 49.

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  • Purified Mouse IgG
  • Purified Mouse IgG
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP01XIYdatasheet pdfdatasheet pdf10 mg
    PMP01X
    PMP01IYdatasheet pdfdatasheet pdf2 mg
    PMP01
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG Formats

    Formats Applications Sizes available
    IgG : Purified IY 10 mg | 2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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