IgG Antibody

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IgG Antibody gallery image 1

Figure A. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11b (MCA1777S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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IgG Antibody gallery image 2

Figure A. PE conjugated Mouse anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on monocytes.

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IgG Antibody gallery image 3

Figure A. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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IgG Antibody gallery image 4

Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 PE (STAR74D649) and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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IgG Antibody gallery image 5

Figure A. FITC conjugated Mouse anti Canine CD8 (MCA1039F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD8 (MCA1039F) and purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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IgG Antibody gallery image 6

Figure A. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG1 FITC isotype control (MCA6004F). Figure B. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 PE (STAR74D649) and Rat anti Canine CD8 FITC (MCA1039F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • IgG Antibody thumbnail image 5
  • IgG Antibody thumbnail image 6
  • Goat anti Mouse IgG:DyLight®649 (Rat Adsorbed)
  • Goat anti Mouse IgG:Dylight®800 (Rat Adsorbed)
  • Goat anti Mouse IgG:Dylight®405 (Rat Adsorbed)
  • Goat anti Mouse IgG:DyLight®549 (Rat Adsorbed)
  • Goat anti Mouse IgG (Rat Adsorbed)
  • Goat anti Mouse IgG:DyLight®488 (Rat Adsorbed)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR74D649GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR74D649GA
    STAR74D800GAF, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    STAR74D800GA
    STAR74D405GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR74D405GA
    STAR74D549GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR74D549GA
    STAR74D488GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR74D488GA
    STAR74E, Fdatasheet pdfdatasheet pdf0.5 mg
    STAR74
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Mouse IgG (Rat Adsorbed) antibody recognizes mouse IgG. Cross-reactivity with rat IgG has been minimised by adsorption.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to DyLight®649 - liquid
      Purified IgG conjugated to DyLight®800 - liquid
      Purified IgG conjugated to DyLight®405 - liquid
      Purified IgG conjugated to DyLight®549 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to DyLight®488 - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Mouse IgG.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      antibody-dependent cellular cytotoxicity
      integral to membrane
      positive regulation of type I hypersensitivity
      immunoglobulin complex, circulating
      positive regulation of type IIa hypersensitivity
      humoral immune response mediated by circulating immunoglobulin
      plasma membrane
      complement activation, classical pathway
      antigen binding
      phagocytosis, engulfment
      positive regulation of phagocytosis
      antibacterial humoral response
      phagocytosis, recognition
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunofluorescence1/1001/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Western Blotting1/50001/20000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunofluorescence

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : DyLight®800 F, IF, WB 0.1 mg
    IgG Antibody : DyLight®405 F, IF 0.1 mg
    IgG Antibody : DyLight®649 F, IF 0.1 mg
    IgG Antibody : DyLight®549 F, IF 0.1 mg
    IgG Antibody : DyLight®488 F, IF 0.1 mg
    IgG Antibody : Purified E, F 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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