Goat anti Mouse IgG antibody recognizes mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues.
Goat IgG shows minimal binding to Fc receptors on rat tissue. We recommend diluting this product in buffer containing 10% normal rat serum to remove any residual cross-reactivity.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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2. Win, TS. et al. (2009) Donor CD4 T cells contribute to cardiac allograft vasculopathy by providing help for autoantibody production.Circ. Heart Fail. 2: 361-369.
3. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.FASEB J. 26 (1): 51-62.
4. Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.J Immunol. 188 (6): 2643-52.
5. Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.Invest Ophthalmol Vis Sci. 53 (11): 7358-69.
6. Gibbings, D.J. et al. (2007) CD8α is expressed by human monocytes and enhances FcγR-dependent responses.BMC Immunol. 8: 12.