Feline Immunodeficiency Virus gp95 antibody | SU1-30
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Mouse anti Feline Immunodeficiency Virus gp95
- Product Type
- Monoclonal Antibody
- Clone
- SU1-30
- Isotype
- IgG1
- Specificity
- Feline Immunodeficiency Virus gp95
- Region
- V3 REGION
Antibody Quality and Performance Guarantee
| Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 recognizes the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU,gp95. The primary receptor for FIV infection of cats is the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion of the CD4+ population. The V3 loop of SU,gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU,gp95 to CXCR4 (Sundstrom et al. 2008). Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 inhibits the sCD134-activated entry of both clade B and clade C FIV strains (de Parseval, A. et al. 2006). Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of sodium azide is recommended prior to use in functional assays. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Functional Assays | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Western Blotting
- MCA2552 detects a band of approximately 95kDa under reducing conditions
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References for Feline Immunodeficiency Virus gp95 antibody
-
DeParseval, A. et al. (2006) Sequential CD134-CXCR4 interactions in feline immunodeficiency virus (FIV): soluble CD134 activates FIV Env for CXCR4-dependent entry and reveals a cryptic neutralization epitope.
J Virol. 80 (6): 3088-91. -
Sundstrom, M. et al. (2008) Mapping of the CXCR4 binding site within variable region 3 of the feline immunodeficiency virus surface glycoprotein.
J Virol. 82 (18): 9134-42. -
Klonjkowski, B. et al. (2009) Gag-specific immune enhancement of lentiviral infection after vaccination with an adenoviral vector in an animal model of AIDS.
Vaccine. 27: 928-39.
Further Reading
-
Willett, B.J. et al. (2006) Differential utilization of CD134 as a functional receptor by diverse strains of feline immunodeficiency virus.
J Virol. 80 (7): 3386-94.
- RRID
- AB_808577
MCA2552
160813
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