Coronavirus antibody | FIPV3-70
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Spleen of cat n○9. (a) The parenchyma appeared quite normal at low magnification with only a few small periarteriolar lymphoplasmacytic infiltrates with macrophages, resembling normal white pulp (arrow). Inset: higher magnification of the infiltrate, no granulomas or vasculitis were clearly evident. (b) Immunolabeled macrophages were present inside the periarteriolar infiltrate (arrow). Inset: higher magnification.
From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Feline infectious peritonitis (FIP), cat. Typical lesions. (a,c,e,g) hematoxylin–eosin; (b,d,f,h) immunohistochemistry for feline coronavirus. (a,b) Chronic interstitial nephritis, kidney. (a) A moderate perivascular lymphoplasmacytic and macrophagic infiltrate were present. (b) Distribution of immunolabeled cells. Inset: higher magnification. (c,d) Granulomatous hepatitis, liver. (c) Multifocal nodular infiltrates with central necrosis. (d) Distribution of the immunolabeled cells in the infiltrates. Inset: High magnification of macrophages with intense cytoplasmic immunolabeling. (e,f) Fibrinous perihepatitis, liver. (e) The black vertical bars indicate the thick band of fibrin along the serosal surface with few aggregates of inflammatory cells. (f) Multifocal distribution of the immunolabeled cells. (g,h) Lung. Diffuse and multifocal interstitial infiltrates (g) associated with immunolabeled cells (h).
From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Large intestine. No lesions are detectable in the mucosa (a), but few superficial columnar epithelial cells show cytoplasmic immunolabeling without associated infiltrates (b). Inset: higher magnification.
From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV).
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 does not recognize Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.
It has been reported that clone FIPV3 recognizes the SARS-Cov-2 core protein (Nardacci et al. 2020).
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 does not recognize Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.
It has been reported that clone FIPV3 recognizes the SARS-Cov-2 core protein (Nardacci et al. 2020).
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Coronavirus cocktail.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Coronavirus antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
- Western Blotting
- Under reducing conditions, MCA2194 detects a specific band of 50-56 kDa that represents the nucleocapsid.
MCA2194 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg |
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Product Specific References
References for Coronavirus antibody
-
Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
J Comp Pathol. 119 (1): 1-14. -
Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
Vet Pathol. 37 (2): 129-36. -
Michimae, Y. et al. (2010) The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan.
J Toxicol Pathol. 23 (2): 99-101. -
Suderman, M.T. et al. (2006) Three-Dimensional Human Bronchial-Tracheal Epithelial Tissue-Like Assemblies (TLAs) as Hosts For Severe Acute Respiratory Syndrome (SARS)-CoV Infection
NASA/TP–2006–213723 -
Vogel, L. et al. (2010) Pathogenic characteristics of persistent feline enteric coronavirus infection in cats.
Vet Res. 41 (5): 71. -
Cony, F.G. et al. (2019) Clinical and pathological aspects of idiopathic pulmonary fibrosis in cats
Pesquisa Veterinária Brasileira. 39 (2): 134-41. -
Stranieri, A. et al. (2020) Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
Pathogens. 9 (10): 852. -
Stranieri, A. et al. (2020) Preliminary investigation on feline coronavirus presence in the reproductive tract of the tom cat as a potential route of viral transmission.
J Feline Med Surg. 22 (2): 178-85.
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