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Coronavirus antibody | FIPV3-70

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Anti Feline Coronavirus Antibody, clone FIPV3-70 thumbnail image 1 Anti Feline Coronavirus Antibody, clone FIPV3-70 thumbnail image 2 Anti Feline Coronavirus Antibody, clone FIPV3-70 thumbnail image 3
Anti Feline Coronavirus Antibody, clone FIPV3-70 gallery image 1
Published customer image:
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Spleen of cat n○9. (a) The parenchyma appeared quite normal at low magnification with only a few small periarteriolar lymphoplasmacytic infiltrates with macrophages, resembling normal white pulp (arrow). Inset: higher magnification of the infiltrate, no granulomas or vasculitis were clearly evident. (b) Immunolabeled macrophages were present inside the periarteriolar infiltrate (arrow). Inset: higher magnification.

From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Feline Coronavirus Antibody, clone FIPV3-70 gallery image 2
Published customer image:
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Feline infectious peritonitis (FIP), cat. Typical lesions. (a,c,e,g) hematoxylin–eosin; (b,d,f,h) immunohistochemistry for feline coronavirus. (a,b) Chronic interstitial nephritis, kidney. (a) A moderate perivascular lymphoplasmacytic and macrophagic infiltrate were present. (b) Distribution of immunolabeled cells. Inset: higher magnification. (c,d) Granulomatous hepatitis, liver. (c) Multifocal nodular infiltrates with central necrosis. (d) Distribution of the immunolabeled cells in the infiltrates. Inset: High magnification of macrophages with intense cytoplasmic immunolabeling. (e,f) Fibrinous perihepatitis, liver. (e) The black vertical bars indicate the thick band of fibrin along the serosal surface with few aggregates of inflammatory cells. (f) Multifocal distribution of the immunolabeled cells. (g,h) Lung. Diffuse and multifocal interstitial infiltrates (g) associated with immunolabeled cells (h).

From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Feline Coronavirus Antibody, clone FIPV3-70 gallery image 3
Published customer image:
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 (MCA2194) used for the demonstration of feline coronovirus infected cells in cat spleen sections by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Large intestine. No lesions are detectable in the mucosa (a), but few superficial columnar epithelial cells show cytoplasmic immunolabeling without associated infiltrates (b). Inset: higher magnification.

From: Stranieri, A.; Scavone, D.; Paltrinieri, S.; Giordano, A.; Bonsembiante, F.; Ferro, S.; Gelain, M.E.; Meazzi, S.; Lauzi, S.
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis.
Pathogens 2020, 9, 852.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Feline Coronavirus

Product Type
Monoclonal Antibody
Clone
FIPV3-70
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2194
Datasheet
C E F IF P* WB
SDS
0.25 mg loader

Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV).
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 does not recognize Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.

It has been reported that clone FIPV3 recognizes the SARS-Cov-2 core protein (Nardacci et al. 2020).

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide
Immunogen
Coronavirus cocktail.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Coronavirus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
Western Blotting
Under reducing conditions, MCA2194 detects a specific band of 50-56 kDa that represents the nucleocapsid.

MCA2194 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Coronavirus antibody

  1. Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
    J Comp Pathol. 119 (1): 1-14.
  2. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  3. Michimae, Y. et al. (2010) The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan.
    J Toxicol Pathol. 23 (2): 99-101.
  4. Suderman, M.T. et al. (2006) Three-Dimensional Human Bronchial-Tracheal Epithelial Tissue-Like Assemblies (TLAs) as Hosts For Severe Acute Respiratory Syndrome (SARS)-CoV Infection
    NASA/TP–2006–213723
  5. Vogel, L. et al. (2010) Pathogenic characteristics of persistent feline enteric coronavirus infection in cats.
    Vet Res. 41 (5): 71.
  6. Cony, F.G. et al. (2019) Clinical and pathological aspects of idiopathic pulmonary fibrosis in cats
    Pesquisa Veterinária Brasileira. 39 (2): 134-41.
  7. Stranieri, A. et al. (2020) Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
    Pathogens. 9 (10): 852.
  8. Stranieri, A. et al. (2020) Preliminary investigation on feline coronavirus presence in the reproductive tract of the tom cat as a potential route of viral transmission.
    J Feline Med Surg. 22 (2): 178-85.

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