Coronavirus antibody | FIPV3-70

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Mouse anti Feline Coronavirus

Product Type
Monoclonal Antibody
Clone
FIPV3-70
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2194 C E F IF P* WB 0.25 mg
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV).
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide
Immunogen
Coronavirus cocktail.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Coronavirus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
ELISA
MCA2194 can be used as a capture reagent in sandwich ELISA assays in combination with MCA2594B as a detection reagent.

Western Blotting
Under reducing conditions, MCA2194 detects a specific band of 50-56 kDa that represents the nucleocapsid.

MCA2194 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F

Product Specific References

References for Coronavirus antibody

  1. Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
    J Comp Pathol. 119 (1): 1-14.
  2. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  3. Michimae, Y. et al. (2010) The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan
    J. Toxicol. Pathol. 23: 99-101.
  4. Suderman, M.T. et al. (2006) Three-Dimensional Human Bronchial-Tracheal Epithelial Tissue-Like Assemblies (TLAs) as Hosts For Severe Acute Respiratory Syndrome (SARS)-CoV Infection
    NASA/TP–2006–213723
  5. Vogel, L. et al. (2010) Pathogenic characteristics of persistent feline enteric coronavirus infection in cats.
    Vet Res. 41 (5): 71.