Coronavirus antibody | FIPV3-70
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 does not recognize Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.
It has been reported that clone FIPV3 recognizes the SARS-Cov-2 core protein (Nardacci et al. 2020).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Coronavirus cocktail.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
Applications of Coronavirus antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- Under reducing conditions, MCA2194 detects a specific band of 50-56 kDa that represents the nucleocapsid.
MCA2194 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)||STAR13B||C E P RE WB||1 mg|
|Goat anti Mouse IgG:FITC (Rat Adsorbed)||STAR70||F||0.5 mg|
Product Specific References
References for Coronavirus antibody
Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
J Comp Pathol. 119 (1): 1-14.
Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
Vet Pathol. 37 (2): 129-36.
Michimae, Y. et al. (2010) The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan.
J Toxicol Pathol. 23 (2): 99-101.
Suderman, M.T. et al. (2006) Three-Dimensional Human Bronchial-Tracheal Epithelial Tissue-Like Assemblies (TLAs) as Hosts For Severe Acute Respiratory Syndrome (SARS)-CoV Infection
Vogel, L. et al. (2010) Pathogenic characteristics of persistent feline enteric coronavirus infection in cats.
Vet Res. 41 (5): 71.
Cony, F.G. et al. (2019) Clinical and pathological aspects of idiopathic pulmonary fibrosis in cats
Pesquisa Veterinária Brasileira. 39 (2): 134-41.
Stranieri, A. et al. (2020) Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
Pathogens. 9 (10): 852.
Stranieri, A. et al. (2020) Preliminary investigation on feline coronavirus presence in the reproductive tract of the tom cat as a potential route of viral transmission.
J Feline Med Surg. 22 (2): 178-85.
Meazzi, S. et al. (2019) Feline gut microbiota composition in association with feline coronavirus infection: A pilot study.
Res Vet Sci. 125: 272-8.
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