Ustekinumab antibody | AbD17829
Antigens were coated at 5 μg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA, the anti-Ustekinumab antibody, clone AbD17829 (20μL from a 2μg/mL solution) was added followed by Mouse anti-V5-tag:HRP (MCA1360P) in HiSPEC buffer (BUF049). Detection was performed using a fluorogenic peroxidase substrate.
IL-23 (1.0 μg/mL) was used as the coating antigen followed by a pre-incubated mixture of the anti-idiotypic antibody (HCA208) titrated into a constant amount of Ustekinumab (0.3 µg/mL). Free Ustekinumab still capable of binding to the plate was detected using Mouse anti Human IgG(Fc):HRP (MCA647P). Data is shown as the mean of three measurements.
Anti-Ustekinumab antibody HCA208 (AbD17829) was coated at 1.0 μg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA in PBST, Ustekinumab spiked into 10% human serum was added. Detection was performed by adding HRP conjugated anti-Ustekinumab antibody HCA210, 2 μg/mL in HiSPEC buffer (BUF049), plus a fluorogenic peroxidase substrate. Data is shown as the mean of three measurements.
Human anti Ustekinumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD17829
- Isotype
- HuCAL Fab monovalent
- Specificity
- Ustekinumab
Antibody Quality and Performance Guarantee
| Human Anti-Ustekinumab Antibody, clone AbD17829 is a paratope specific, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug ustekinumab and inhibits binding to its target. The antibody can be used to measure the level of free ustekinumab and biosimilar products in bioanalytical and patient monitoring assays. A pair of anti-ustekinumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as a capture antibody, paired with an HRP conjugated Anti-Ustekinumab Antibody in full immunoglobulin format, clone AbD17827_hIgG1 (HCA210) as the detection antibody. Ustekinumab (Stelara) is a fully human monoclonal antibody drug (IgG1/kappa) that has been approved in US, Canada, and Europe for the treatment of plaque psoriasis and psoriatic arthritis. The FDA approved the medication for the treatment of moderate-severe Crohn's disease in 2016. The drug blocks interleukin IL-12 and IL-23 by binding with high affinity and specificity to the p40 subunit, preventing the binding of these two cytokines to T-lymphocytes, and thereby disrupting the proinflammatory pathway. View a summary of all anti-ustekinumab antibodies |
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- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK).
- Preparation
- StrepTactin affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Ustekinumab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Ustekinumab antibody, clone AbD17829 is KD = 2.8 nM as measured by real time, label free molecular interaction analysis on immobilized ustekinumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
This antibody contains Strep-tag and will react with streptavidin. - ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with HCA210P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti V5-Tag:Biotin | ||||||
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti V5-Tag:HRP | ||||||
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Strep-Tag Classic:HRP | ||||||
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C * E | 0.2 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP | ||||||
- Synonyms
- Stelara®
HCA208
152187
157800
168868
1808
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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