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Histidine Tag antibody | AD1.1.10

Mouse anti Histidine Tag:HRP

Product Type
Monoclonal Antibody
Histidine Tag

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.

In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
PAX6 transcription factor linked to histidine tag.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
For research purposes only
12 months from date of despatch
His-tag is a trademark of EMD Biosciences

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/1000
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for Histidine Tag antibody

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