CD45 antibody | OX-1









Mouse anti Rat CD45:Alexa Fluor® 488
Mouse anti Rat CD45:Alexa Fluor® 647
Mouse anti Rat CD45:FITC
Mouse anti Rat CD45
Mouse anti Rat CD45:RPE
Mouse anti Rat CD45:RPE-Alexa Fluor® 750
Mouse anti Rat CD45:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- OX-1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA43A488 | F | 100 Tests/1ml |
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MCA43A647 | F | 100 Tests/1ml |
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MCA43FA | F | 50 µg |
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MCA43FT | F | 0.1 mg |
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MCA43FB | F | 0.5 mg |
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MCA43GA | C* F IF | 0.1 mg |
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MCA43R | C* F IF | 0.25 mg |
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MCA43G | C* F IF | 1 mg |
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MCA43PE | F | 100 Tests |
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MCA43P750 | F | 100 Tests/1ml |
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MCA43A700 | F | 100 Tests/1ml |
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Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
This product is routinely tested in flow cytometry on rat splenocytes
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488-liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04157
- Entrez Gene
- Ptprc
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD45 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat Pack Size: 50 µg, 0.1 mg 1/5 Pack Size: 0.5 mg |
1/10 Pack Size: 50 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
|
Flow Cytometry | 1/100 | ||
Immunofluorescence | |||
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Flow Cytometry | Neat | ||
Flow Cytometry | |||
Flow Cytometry | Neat | 1/5 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA1209A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
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Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE-Alexa Fluor® 750 | MCA1209P750 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA1209A700 | F | 100 Tests/1ml |
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Product Specific References
References for CD45 antibody
-
Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Eur J Immunol. 9 (2): 155-9. -
Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73. -
Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
Clin Exp Immunol. 99 (2): 283-8. -
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
Int Immunol. 12 (2): 169-76. -
Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
Eur J Immunol. 8 (12): 832-9. -
Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
J Immunol. 163 (1): 71-6. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Schupp, N. et al. (2011) Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
FASEB J. 25 (3): 968-78. -
Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
Am J Pathol. 158: 407-17. -
Jeong, H.K. et al (2010) Inflammatory responses are not sufficient to cause delayed neuronal death in ATP-induced acute brain injury.
PLoS One. 5: e13756. -
Leonardo, C.C. et al. (2009) Inhibition of gelatinase activity reduces neural injury in an ex vivo model of hypoxia-ischemia.
Neuroscience. 160: 755-66. -
Markusic, D.M. et al. (2010) Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.
PLoS One. 5: e9974. -
Vaschetto, R. et al. (2010) Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
Crit Care. 14: R45. -
Ladhoff, J. et al. (2010) Immune privilege of endothelial cells differentiated from endothelial progenitor cells.
Cardiovasc Res. 88: 121-9. -
Yao, Y. et al. (2016) Alendronate Attenuates Spinal Microglial Activation and Neuropathic Pain.
J Pain. 17 (8): 889-903. -
Wang, C. et al. (2015) Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD.
Int Braz J Urol. 41 (4): 764-72. -
Tanner, D.C. et al. (2015) cFLIP is critical for oligodendrocyte protection from inflammation.
Cell Death Differ. 22 (9): 1489-501. -
Runesson, E. et al. (2015) Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
BMC Musculoskelet Disord. 16: 212. -
Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
J Neuroinflammation. 16 (1): 93. -
Pilipović, I. et al. (2019) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
Neurobiol Dis. Nov 2 [Epub ahead of print].
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