CD45 antibody | OX-1
Mouse anti Rat CD45 antibody, clone OX-1 (MCA43R) used for illustration of leukocytes in rat brain by immunohistofluorescence.
Image caption:
Infiltration of monocytes in ATP-injected SNpc.
Sections were obtained 2 d after ATP injection, and double-labeled with Iba-1 and CD45 antibodies. Round Iba-1+ cells were CD45+ (arrows) whereas ramified Iba-1+ cells were CD45- (arrowheads).Scale Bar =50μm (B).
From: Jeong H-K, Ji K-m, Kim B, Kim J, Jou I, Joe E.H. (2010)
Inflammatory Responses Are Not Sufficient to Cause Delayed Neuronal Death in ATP-Induced Acute Brain Injury.
PLoS ONE 5(10): e13756.
RPE-AlexaFluor®750 conjugated Mouse anti Rat CD45 antibody, clone OX-1 (MCA43P750) used for the identification of macrophage populations by flow cytometry.
Image caption:
Flow cytometry performed seven days post-injury. Activated macrophages were distinguished by their expression of CD11b and CD45 (a). No difference was observed in the number of CD11b+CD45HIGH macrophages between treatment groups (b). *P <0.05 (Tukey's Test); error bars represent ± SEM; n = 3 for Controls and 4 for all other groups.
From: Hellenbrand DJ, Reichl KA, Travis BJ, Filipp ME, Khalil AS, Pulito DJ, Gavigan AV, Maginot ER, Arnold MT, Adler AG, Murphy WL, Hanna AS.
Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
J Neuroinflammation. 2019 Apr 30;16(1):93.
doi: 10.1186/s12974-019-1479-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD45, clone OX-1 (MCA43G) used to label microglia in rat brain by immunohistochemistry on formalin fixed cryostections.
Image caption:
Quantification of CD45+ leukocytes in the striatum of the grafted rats. (C, D) Bright field images showing CD45+ cells density and morphology around the graft and their quantification respectively in the striatum of the rats received either saline, exendin-4 or liraglutide and ± LD. ** p <0.01, *** p <0.001, **** p <0.0001. Scale bar (A,C) = 100 μm.
From:Elabi OF, Davies JS, Lane EL.
L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
Int J Mol Sci. 2021 Nov 16;22(22):12346.
doi: 10.3390/ijms222212346.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-phycoerythrin conjugated Mouse anti Rat CD45 antibody, clone OX-1 (MCA43PE) used to characterise rat tendon stem/progenitor cells (TSPCs) by flow cytometry.
Image caption:
Isolation and Characterization of TSPCs.
(C) Flow cytometry analysis of the expression of cell surface makers related to stem cells and hematopoietic cells.
From: Yang Q, Li J, Su W, Yu L, Li T, Wang Y, Zhang K, Wu Y, Wang L.
Electrospun aligned poly(ε-caprolactone) nanofiber yarns guiding 3D organization of tendon stem/progenitor cells in tenogenic differentiation and tendon repair.
Front Bioeng Biotechnol. 2022 Aug 30;10:960694.
doi: 10.3389/fbioe.2022.960694.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F IF C ResetMouse anti Rat CD45:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- OX-1
- Isotype
- IgG1
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This product is routinely tested in flow cytometry on rat splenocytes |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
References for CD45 antibody
-
Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
Eur J Immunol. 8 (12): 832-9. -
Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Eur J Immunol. 9 (2): 155-9. -
Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73. -
Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
Clin Exp Immunol. 99 (2): 283-8. -
Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
J Immunol. 163 (1): 71-6. -
Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
Int Immunol. 12 (2): 169-76. -
Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
Am J Pathol. 158: 407-17. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6.
View The Latest Product References
-
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Kurozumi, K. et al. (2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.
J Natl Cancer Inst. 99: 1768-81. -
Leonardo, C.C. et al. (2009) Inhibition of gelatinase activity reduces neural injury in an ex vivo model of hypoxia-ischemia.
Neuroscience. 160: 755-66. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Jeong, H.K. et al (2010) Inflammatory responses are not sufficient to cause delayed neuronal death in ATP-induced acute brain injury.
PLoS One. 5: e13756. -
Markusic, D.M. et al. (2010) Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.
PLoS One. 5: e9974. -
Vaschetto, R. et al. (2010) Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
Crit Care. 14: R45. -
Ladhoff, J. et al. (2010) Immune privilege of endothelial cells differentiated from endothelial progenitor cells.
Cardiovasc Res. 88: 121-9. -
Schupp, N. et al. (2011) Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
FASEB J. 25 (3): 968-78. -
Runesson, E. et al. (2015) Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
BMC Musculoskelet Disord. 16: 212. -
Tanner, D.C. et al. (2015) cFLIP is critical for oligodendrocyte protection from inflammation.
Cell Death Differ. 22 (9): 1489-501. -
Wang, C. et al. (2015) Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD.
Int Braz J Urol. 41 (4): 764-72. -
Yao, Y. et al. (2016) Alendronate Attenuates Spinal Microglial Activation and Neuropathic Pain.
J Pain. 17 (8): 889-903. -
Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
Stem Cells Dev. 27 (16): 1109-24. -
Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
J Neuroinflammation. 16 (1): 93. -
Pilipović, I. et al. (2020) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
Neurobiol Dis. 134: 104665. -
Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
Toxicol Sci. kfz172. -
Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
Regen Ther. 14: 1-10. -
Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
Exp Neurol. 336: 113531. -
Elabi, O.F. et al. (2021) L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
Int J Mol Sci. 22(22):12346. -
Hou, Y. et al. (2021) Pseudoginsenoside-F11 promotes functional recovery after transient cerebral ischemia by regulating the microglia/macrophage polarization in rats.
Int Immunopharmacol. 99: 107896. -
Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
PLoS One. 17 (8): e0272697. -
Yang, Q. et al. (2022) Electrospun aligned poly(ε-caprolactone) nanofiber yarns guiding 3D organization of tendon stem/progenitor cells in tenogenic differentiation and tendon repair.
Front Bioeng Biotechnol. 10: 960694.
- Synonyms
- LCA
- RRID
- AB_321197
- UniProt
- P04157
- Entrez Gene
- Ptprc
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- View More GO Terms
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
MCA43A647
150533
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