CD3 antibody | 1F4
Mouse anti Rat CD3 antibody, clone 1F4 (MCA772) used for the identification of CD3 expressing lymphocytes in rat spleen by immunohistochemistry on formalin fixed, paraffin embedded tissue sections following EDTA mediated antigen retrieval.
Image caption:
CD3/ 1F4/ AbD serotec/ MCA772, Spleen/ Rat.
From: Furukawa S, Nagaike M, Ozaki K.
Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 2017 Jan;30(1):79-107.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD3:Alexa Fluor® 647
CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
Mouse anti Rat CD3:Biotin
CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
Mouse anti Rat CD3:FITC
CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
Mouse anti Rat CD3
CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
Mouse anti Rat CD3:Alexa Fluor® 700
CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
- Product Type
- Monoclonal Antibody
- Clone
- 1F4
- Isotype
- IgM
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA772A647 | F | 100 Tests/1ml |
|
|
| MCA772B | F | 0.1 mg |
|
|
| MCA772F | F | 0.1 mg |
|
|
| MCA772 | C F IP P* | 0.2 mg |
|
|
| MCA772GA | C F IP P* | 0.1 mg |
|
|
| MCA772A700 | F | 100 Tests/1ml |
|
Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.
Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgM conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgM conjugated to Biotin - liquid
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgM - liquid
- Product Form
- Purified IgM conjugated to Alexa Fluor® 700 - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
- Approx. Protein Concentrations
- IgM concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.2 mgIgM concentration 1.0 mg/mlPack Size: 0.1 mgIgM concentration 1 mg/ml
- Approx. Protein Concentrations
- IgM concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19377
- Q64159
- Entrez Gene
- Cd3d
- Cd3g
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0045059 positive thymic T cell selection
- GO:0007163 establishment or maintenance of cell polarity
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- GO:0046982 protein heterodimerization activity
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/25 | |
| Immunohistology - Frozen | 1/10 | 1/25 | |
| Immunohistology - Paraffin 1 | 1/10 | ||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
- 1 This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgM:HRP | 102005 | C E IB | 1 ml |
|
|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
Product Specific References
References for CD3 antibody
-
Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
J Immunol. 142 (8): 2791-5. -
Nicolls, M.G. et al. (1992) Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.
Transplantation 55: 459-68. -
McKechnie NM et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J Infect Dis. 176 (5): 1334-43. -
Lohwasser, C. et al. (2009) Role of the receptor for advanced glycation end products in hepatic fibrosis.
World J Gastroenterol. 15: 5789-98. -
Beck, K.D. et al (2010) Quantitative analysis of cellular inflammation after traumatic spinal cord injury: evidence for a multiphasic inflammatory response in the acute to chronic environment.
Brain. 133: 433-47. -
Candolfi, M. et al. (2007) Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression.
J Neurooncol. 85: 133-48. -
Sanchez-Guajardo, V. et al. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
PLoS One. 5: e8784. -
Echeverry, S. et al. (2011) Peripheral Nerve Injury Alters Blood-Spinal Cord Barrier Functional and Molecular Integrity through a Selective Inflammatory Pathway.
J Neurosci. 31: 10819-28. -
Takahashi Y et al. (2016) Rituximab protects podocytes and exerts anti-proteinuric effects in rat adriamycin-induced nephropathy independent of B-lymphocytes.
Nephrology (Carlton). Feb 2. [Epub ahead of print] -
Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
Int J Clin Exp Pathol 10(5): 5252-62.
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