CD3 antibody | 1F4
Mouse anti Rat CD3 antibody, clone 1F4 (MCA772) used for the identification of CD3 expressing lymphocytes in rat spleen by immunohistochemistry on formalin fixed, paraffin embedded tissue sections following EDTA mediated antigen retrieval.
Image caption:
CD3/ 1F4/ AbD serotec/ MCA772, Spleen/ Rat.
From: Furukawa S, Nagaike M, Ozaki K.
Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 2017 Jan;30(1):79-107.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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F P ResetMouse anti Rat CD3
- Product Type
- Monoclonal Antibody
- Clone
- 1F4
- Isotype
- IgM
- Specificity
- CD3
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Antibody Quality and 
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| Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA. |
- Target Species
- Rat
- Product Form
- Purified IgM - liquid
- Preparation
- MCA772: Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
- MCA772GA: Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
- Approx. Protein Concentrations
- Pack Size: 0.2 mgIgM concentration 1.0 mg/mlPack Size: 0.1 mgIgM concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/25 |
| Immunohistology - Frozen | |
1/10 | 1/25 |
| Immunohistology - Paraffin 1 | |
1/10 | |
| Immunoprecipitation | |
- 1 This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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References for CD3 antibody
-
Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
J Immunol. 142 (8): 2791-5. -
Nicolls, M.G. et al. (1992) Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.
Transplantation 55: 459-68. -
McKechnie NM et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J Infect Dis. 176 (5): 1334-43. -
Lohwasser, C. et al. (2009) Role of the receptor for advanced glycation end products in hepatic fibrosis.
World J Gastroenterol. 15: 5789-98. -
Beck, K.D. et al (2010) Quantitative analysis of cellular inflammation after traumatic spinal cord injury: evidence for a multiphasic inflammatory response in the acute to chronic environment.
Brain. 133: 433-47. -
Candolfi, M. et al. (2007) Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression.
J Neurooncol. 85: 133-48. -
Sanchez-Guajardo, V. et al. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
PLoS One. 5: e8784. -
Echeverry, S. et al. (2011) Peripheral Nerve Injury Alters Blood-Spinal Cord Barrier Functional and Molecular Integrity through a Selective Inflammatory Pathway.
J Neurosci. 31: 10819-28.
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- RRID
- AB_321258
- UniProt
- P19377
- Q64159
- Entrez Gene
- Cd3d
- Cd3g
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0045059 positive thymic T cell selection
- GO:0007163 establishment or maintenance of cell polarity
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- GO:0046982 protein heterodimerization activity
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