CD3 antibody | 1F4

Mouse anti Rat CD3

Product Type
Monoclonal Antibody
Clone
1F4
Isotype
IgM
Specificity
CD3

Product Code Applications Pack Size List Price Your Price Qty
MCA772
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 0.2 mg loader
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loader
MCA772GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 0.1 mg loader
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loader
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Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.

Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Target Species
Rat
Product Form
Purified IgM - liquid
Preparation
MCA772: Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
MCA772GA: Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
Approx. Protein Concentrations
Pack Size: 0.2 mg
IgM concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgM concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Immunohistology - Frozen 1/10 1/25
Immunohistology - Paraffin 1 1/10
Immunoprecipitation
  1. 1 This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD3 antibody

  1. Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
    J Immunol. 142 (8): 2791-5.
  2. Nicolls, M.G. et al. (1992) Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.
    Transplantation 55: 459-68.
  3. McKechnie NM et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
    J Infect Dis. 176 (5): 1334-43.
  4. Lohwasser, C. et al. (2009) Role of the receptor for advanced glycation end products in hepatic fibrosis.
    World J Gastroenterol. 15: 5789-98.
  5. Beck, K.D. et al (2010) Quantitative analysis of cellular inflammation after traumatic spinal cord injury: evidence for a multiphasic inflammatory response in the acute to chronic environment.
    Brain. 133: 433-47.
  6. Candolfi, M. et al. (2007) Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression.
    J Neurooncol. 85: 133-48.
  7. Sanchez-Guajardo, V. et al. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
    PLoS One. 5: e8784.
  8. Echeverry, S. et al. (2011) Peripheral Nerve Injury Alters Blood-Spinal Cord Barrier Functional and Molecular Integrity through a Selective Inflammatory Pathway.
    J Neurosci. 31: 10819-28.
  9. View The Latest Product References
  10. Takahashi Y et al. (2016) Rituximab protects podocytes and exerts anti-proteinuric effects in rat adriamycin-induced nephropathy independent of B-lymphocytes.
    Nephrology (Carlton). Feb 2. [Epub ahead of print]
  11. Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
    Int J Clin Exp Pathol 10(5): 5252-62.

Immunohistology - Frozen

Immunohistology - Paraffin

RRID
AB_321258
UniProt
P19377
Q64159
Entrez Gene
Cd3d
Cd3g
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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