CD3 antibody | 1F4

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Mouse anti Rat CD3:Alexa Fluor® 647

CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.

Mouse anti Rat CD3:Biotin

CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.

Mouse anti Rat CD3:FITC

CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.

Mouse anti Rat CD3

CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.

Mouse anti Rat CD3:Alexa Fluor® 700

CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.

Product Type
Monoclonal Antibody
Clone
1F4
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA772A647 F 100 Tests/1ml
MCA772B F 0.1 mg
MCA772FA F 50 µg
MCA772F F 0.1 mg
MCA772GA C F IP P* 0.1 mg
MCA772 C F IP P* 0.2 mg
MCA772A700 F 100 Tests/1ml
Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.

Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Product Details

Target Species
Rat
Product Form
Purified IgM conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgM conjugated to Biotin - liquid
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Product Form
Purified IgM conjugated to Alexa Fluor® 700 - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
Approx. Protein Concentrations
IgM concentration 0.05 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgM concentration 1 mg/ml
Pack Size: 0.2 mg
IgM concentration 1.0 mg/ml
Approx. Protein Concentrations
IgM concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.2 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P19377 Related reagents
Q64159 Related reagents
Entrez Gene
Cd3d Related reagents
Cd3g Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/5 Pack Size: 50 µg
Neat Pack Size: 0.1 mg
Flow Cytometry 1/10 1/25
Immunohistology - Frozen 1/10 1/25
Immunohistology - Paraffin 1 1/10
Immunoprecipitation
Flow Cytometry Neat
  1. 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgM:HRP 102005 1 ml C E IB
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F

Application Based External Images

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD3 antibody

  1. Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.
    J Immunol. 142 (8): 2791-5.
  2. Nicolls, M.G. et al. (1992) Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.
    Transplantation 55: 459-68.
  3. McKechnie NM et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
    J Infect Dis. 176 (5): 1334-43.
  4. Lohwasser, C. et al. (2009) Role of the receptor for advanced glycation end products in hepatic fibrosis.
    World J Gastroenterol. 15: 5789-98.
  5. Beck, K.D. et al (2010) Quantitative analysis of cellular inflammation after traumatic spinal cord injury: evidence for a multiphasic inflammatory response in the acute to chronic environment.
    Brain. 133: 433-47.
  6. Candolfi, M. et al. (2007) Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression.
    J Neurooncol. 85: 133-48.
  7. Sanchez-Guajardo, V. et al. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
    PLoS One. 5: e8784.
  8. Echeverry, S. et al. (2011) Peripheral Nerve Injury Alters Blood-Spinal Cord Barrier Functional and Molecular Integrity through a Selective Inflammatory Pathway.
    J Neurosci. 31: 10819-28.
  9. Takahashi Y et al. (2016) Rituximab protects podocytes and exerts anti-proteinuric effects in rat adriamycin-induced nephropathy independent of B-lymphocytes.
    Nephrology (Carlton). Feb 2. [Epub ahead of print]
  10. Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
    Int J Clin Exp Pathol 10(5): 5252-62.

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