Mouse anti Rabbit T Cells and Neutrophils antibody, clone RPN3/57 recognizes a subset of T cells (but not RL-5 cell line) and neutrophils by flow cytometry. Gives good staining of T-dependent areas in lymphoid tissue and also of infiltrating neutrophils by immunohistochemistry.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Rabbit Peritoneal Neutrophils.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from innunized Balb/c mice were fuzed with cells of the 653WMB plamacytoma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Thymocytes/Neutrophils/T Cells/Platelets antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for Thymocytes/Neutrophils/T Cells/Platelets antibody
Wilkinson, J.M. et al. (1992) Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies.
Immunology. 76 (4): 625-30.
De Meyer, I. et al. (2011) Inhibition of inositol monophosphatase by lithium chloride induces selective macrophage apoptosis in atherosclerotic plaques.
Br J Pharmacol. 162: 1410-23.
Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
Stroke. 39: 3378-88.
O'Blenes, S.B. et al. (2000) Gene transfer of the serine elastase inhibitor elafin protects against vein graft degeneration.
Circulation. 102 (19 Suppl 3): III289-95.
Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
Arterioscler Thromb Vasc Biol. 20: 2553-8.
Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
J Appl Physiol. 95: 969-76.
Welt, F.G. et al. (2003) Leukocyte recruitment and expression of chemokines following different forms of vascular injury.
Vasc Med. 8: 1-7.
St Pierre Schneider, B. (2002) CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle.
Muscle Nerve. 25: 837-44.
Ma, X. and O'Brien, E.R. (2004) Antagonism of the alpha4 integrin subunit attenuates the acute inflammatory response to stent implantation yet is insufficient to prevent late intimal formation.
J Leukoc Biol. 75: 1016-21.
Hickling, K.G. et al. (1998) Extreme hypoventilation reduces ventilator-induced lung injury during ventilation with low positive end-expiratory pressure in saline-lavaged rabbits.
Crit Care Med. 26: 1690-7.
Bhardwaj, S. et al. (2005) VEGF-A, VEGF-D and VEGF-D(DeltaNDeltaC) induced intimal hyperplasia in carotid arteries.
Eur J Clin Invest. 35: 669-76.
Herold, J. et al. (2004) Transplantation of monocytes: a novel strategy for in vivo augmentation of collateral vessel growth.
Hum Gene Ther. 15: 1-12.
Li, J.M. et al. (2008) Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model.
J Vasc Surg. 47: 1048-57.
Baetta, R. and Corsini, A. (2010) Role of polymorphonuclear neutrophils in atherosclerosis: current state and future perspectives.
Atherosclerosis. 210: 1-13.
Barolet, A.W. et al. (2001) Arterial elastase activity after balloon angioplasty and effects of elafin, an elastase inhibitor.
Arterioscler Thromb Vasc Biol. 21: 1269-74.
Cheema, A.N. et al. (2003) Effects of intravascular cryotherapy on vessel wall repair in a balloon-injured rabbit iliac artery model.
Cardiovasc Res. 59: 222-33.
Li, J.M. et al. (2004) Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury.
J Vasc Surg. 39: 1074-83.
Song, Y. et al. (2012) Effects on contralateral muscles after unilateral electrical muscle stimulation and exercise.
PLoS One. 7(12):e52230.
Song, Y. et al. (2013) Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 14: 134.