Thymocytes/Neutrophils/T Cells/Platelets antibody | RPN3/57

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Mouse anti Rabbit T Cells and Neutrophils

Product Type
Monoclonal Antibody
Clone
RPN3/57
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA805GA C F IF 0.1 mg
Mouse anti Rabbit T Cells and Neutrophils antibody, clone RPN3/57 recognizes a subset of T cells (but not RL-5 cell line) and neutrophils by flow cytometry. Gives good staining of T-dependent areas in lymphoid tissue and also of infiltrating neutrophils by immunohistochemistry.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit Peritoneal Neutrophils.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from innunized Balb/c mice were fuzed with cells of the 653WMB plamacytoma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Thymocytes/Neutrophils/T Cells/Platelets antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for Thymocytes/Neutrophils/T Cells/Platelets antibody

  1. Wilkinson, J.M. et al. (1992) Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies.
    Immunology. 76 (4): 625-30.
  2. De Meyer, I. et al. (2011) Inhibition of inositol monophosphatase by lithium chloride induces selective macrophage apoptosis in atherosclerotic plaques.
    Br J Pharmacol. 162: 1410-23.
  3. Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
    Stroke. 39: 3378-88.
  4. O'Blenes, S.B. et al. (2000) Gene transfer of the serine elastase inhibitor elafin protects against vein graft degeneration.
    Circulation. 102 (19 Suppl 3): III289-95.
  5. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
    Arterioscler Thromb Vasc Biol. 20: 2553-8.
  6. Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    J Appl Physiol. 95: 969-76.
  7. Welt, F.G. et al. (2003) Leukocyte recruitment and expression of chemokines following different forms of vascular injury.
    Vasc Med. 8: 1-7.
  8. St Pierre Schneider, B. (2002) CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle.
    Muscle Nerve. 25: 837-44.
  9. Ma, X. and O'Brien, E.R. (2004) Antagonism of the alpha4 integrin subunit attenuates the acute inflammatory response to stent implantation yet is insufficient to prevent late intimal formation.
    J Leukoc Biol. 75: 1016-21.
  10. Hickling, K.G. et al. (1998) Extreme hypoventilation reduces ventilator-induced lung injury during ventilation with low positive end-expiratory pressure in saline-lavaged rabbits.
    Crit Care Med. 26: 1690-7.
  11. Bhardwaj, S. et al. (2005) VEGF-A, VEGF-D and VEGF-D(DeltaNDeltaC) induced intimal hyperplasia in carotid arteries.
    Eur J Clin Invest. 35: 669-76.
  12. Herold, J. et al. (2004) Transplantation of monocytes: a novel strategy for in vivo augmentation of collateral vessel growth.
    Hum Gene Ther. 15: 1-12.
  13. Li, J.M. et al. (2008) Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model.
    J Vasc Surg. 47: 1048-57.
  14. Baetta, R. and Corsini, A. (2010) Role of polymorphonuclear neutrophils in atherosclerosis: current state and future perspectives.
    Atherosclerosis. 210: 1-13.
  15. Barolet, A.W. et al. (2001) Arterial elastase activity after balloon angioplasty and effects of elafin, an elastase inhibitor.
    Arterioscler Thromb Vasc Biol. 21: 1269-74.
  16. Cheema, A.N. et al. (2003) Effects of intravascular cryotherapy on vessel wall repair in a balloon-injured rabbit iliac artery model.
    Cardiovasc Res. 59: 222-33.
  17. Li, J.M. et al. (2004) Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury.
    J Vasc Surg. 39: 1074-83.
  18. Song, Y. et al. (2012) Effects on contralateral muscles after unilateral electrical muscle stimulation and exercise.
    PLoS One. 7(12):e52230.
  19. Song, Y. et al. (2013) Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
    BMC Musculoskelet Disord. 14: 134.