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Thymocytes/Neutrophils/T Cells/Platelets antibody | RPN3/57

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Anti Rabbit T Cells and Neutrophils Antibody, clone RPN3/57 thumbnail image 1 Anti Rabbit T Cells and Neutrophils Antibody, clone RPN3/57 thumbnail image 2
Anti Rabbit T Cells and Neutrophils Antibody, clone RPN3/57 gallery image 1
Published customer image:
Muscle cross-sections from the non-exercised soleus muscle after 6w of EMS/E (A, B, D–G) and from the exercised side of the gastrocnemius muscle (C). The sections A–C are stained for demonstration of neutrophils/T-lymphocytes (mAb MCA805G). The framed region in (A) is in higher magnification in (B) and the parallel section to the framed region stained for H&E is in the inset (B). The figures show a large number of immuno-reactive cells (arrows) in the connective tissue (A–C). Stars show corresponding muscle fiber in (A) and (B). Figure D show infiltration of inflammatory cells in a necrotic fiber stained with H&E and figure E show the corresponding necrotic fiber (asterix) stained with mAb M0814 against CD68 (macrophages). Figure F show eosinophils (mAb MAB1087) infiltrating a muscle fiber (arrow) In (G), eosinophils (arrows) are stained with H&E in the extracellular matrix of another region of the muscle sample. (Original magnification; A ×100, B–E ×200, F and G x315).

From: Song Y, Forsgren S, Yu J, Lorentzon R, Stål PS (2012) Effects on Contralateral Muscles after Unilateral Electrical Muscle Stimulation and Exercise. PLoS ONE 7(12): e52230.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rabbit T Cells and Neutrophils Antibody, clone RPN3/57 gallery image 2
Published customer image:
Double-staining for tachykinin/white blood cell markers. Sections of soleus muscle tissue of the 6w group, non-exercised side (A-C) and section of gastrocnemius muscle tissue of the 1w group, exercised side (D,E). Double stainings for tachykinin (sc-14104) (green) and neutrophil/T-cell marker (MCA805G) (yellowish) are shown in (A) and (D,E). In (B), double staining for tachykinin (green) and macrophage marker (CD68) (yellowish) is shown and in (C), double staining for tachykinin and eosinophil marker (yellowish) is demonstrated. A white blood cell shows immunoreactivity for the neutrophil/T-cell marker (arrow) but does not show tachykinin-like immunoreactivity (A). There is on the other hand tachykinin-like immunoreactivity (green) in the smooth muscle of a vessel wall (asterisk); the sign (#) depicts debris material within the lumen of the blood vessel (A). There are unspecific reactions in the endothelium of the vessel wall (A). Note that tachykinin-like immunoreactivity (green) can be seen in the macrophage (arrowhead) in (B). There is a blood vessel, in which wall there is only tachykinin-like immunoreactivity (asterisk) (B). There is also tachykinin-like immunoreactivity in eosinophils (arrowheads) (C). Concerning (D,E): The occurrence of reactions for white blood cells after processing for MCA805G is shown in (D) (arrows). The combination of reactions for tachykinin/DAPI is shown in (E). The same cells as indicated in (D) are indicated in (E) (arrows). Note that the cells are not exhibiting tachykinin-like immunoreactivity. There is on the other hand tachykinin-like immunoreactivity in the vessel wall in (E) (asterisk). The reaction for MCA805G is masked in (E). Magnification x315.

From: Song, Y. et al. 2013 Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord. 2013. 14:134.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Rabbit T Cells and Neutrophils

Product Type
Monoclonal Antibody
Clone
RPN3/57
Isotype
IgG1
Specificity
Thymocytes/Neutrophils/T Cells/Platelets

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Product Code Applications Pack Size List Price Your Price Qty
MCA805GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
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Mouse anti Rabbit T Cells and Neutrophils antibody, clone RPN3/57 recognizes a subset of T cells (but not RL-5 cell line) and neutrophils by flow cytometry. Gives good staining of T-dependent areas in lymphoid tissue and also of infiltrating neutrophils by immunohistochemistry.

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit Peritoneal Neutrophils.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from innunized Balb/c mice were fuzed with cells of the 653WMB plamacytoma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Wilkinson, J.M. et al. (1992) Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies.
    Immunology. 76 (4): 625-30.

References for Thymocytes/Neutrophils/T Cells/Platelets antibody

  1. De Meyer, I. et al. (2011) Inhibition of inositol monophosphatase by lithium chloride induces selective macrophage apoptosis in atherosclerotic plaques.
    Br J Pharmacol. 162: 1410-23.
  2. Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
    Stroke. 39: 3378-88.
  3. O'Blenes, S.B. et al. (2000) Gene transfer of the serine elastase inhibitor elafin protects against vein graft degeneration.
    Circulation. 102 (19 Suppl 3): III289-95.
  4. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
    Arterioscler Thromb Vasc Biol. 20: 2553-8.
  5. Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    J Appl Physiol. 95: 969-76.
  6. Welt, F.G. et al. (2003) Leukocyte recruitment and expression of chemokines following different forms of vascular injury.
    Vasc Med. 8: 1-7.
  7. St Pierre Schneider, B. (2002) CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle.
    Muscle Nerve. 25: 837-44.
  8. Ma, X. and O'Brien, E.R. (2004) Antagonism of the alpha4 integrin subunit attenuates the acute inflammatory response to stent implantation yet is insufficient to prevent late intimal formation.
    J Leukoc Biol. 75: 1016-21.
    9. View The Latest Product References
  9. Hickling, K.G. et al. (1998) Extreme hypoventilation reduces ventilator-induced lung injury during ventilation with low positive end-expiratory pressure in saline-lavaged rabbits.
    Crit Care Med. 26: 1690-7.
  10. Bhardwaj, S. et al. (2005) VEGF-A, VEGF-D and VEGF-D(DeltaNDeltaC) induced intimal hyperplasia in carotid arteries.
    Eur J Clin Invest. 35: 669-76.
  11. Herold, J. et al. (2004) Transplantation of monocytes: a novel strategy for in vivo augmentation of collateral vessel growth.
    Hum Gene Ther. 15: 1-12.
  12. Li, J.M. et al. (2008) Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model.
    J Vasc Surg. 47: 1048-57.
  13. Barolet, A.W. et al. (2001) Arterial elastase activity after balloon angioplasty and effects of elafin, an elastase inhibitor.
    Arterioscler Thromb Vasc Biol. 21: 1269-74.
  14. Cheema, A.N. et al. (2003) Effects of intravascular cryotherapy on vessel wall repair in a balloon-injured rabbit iliac artery model.
    Cardiovasc Res. 59: 222-33.
  15. Li, J.M. et al. (2004) Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury.
    J Vasc Surg. 39: 1074-83.
  16. Song, Y. et al. (2012) Effects on contralateral muscles after unilateral electrical muscle stimulation and exercise.
    PLoS One. 7(12):e52230.
  17. Song, Y. et al. (2013) Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
    BMC Musculoskelet Disord. 14: 134.

Further Reading

  1. Baetta, R. and Corsini, A. (2010) Role of polymorphonuclear neutrophils in atherosclerosis: current state and future perspectives.
    Atherosclerosis. 210: 1-13.

MCA805GA

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