CD8 antibody | 12.C7
Mouse anti Rabbit CD8:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 12.C7
- Isotype
- IgG1
- Specificity
- CD8
Antibody Quality and Performance Guarantee
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.
Image caption:
Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.
In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry. A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis). B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P<0.05; **P<0.005, *** P<0.0001). In the left column the following symbols were used: small closed triangle, MR17/1 and IR17/1; small open square, MR17/2 and IR17/2; small closed circle, MR20 and IR20; large open circle, MR24 and IR24.
From: Dewals B, Boudry C, Farnir F, Drion P-V, Vanderplasschen A (2008)
Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8+ T Cells Supporting a Latent Infection.
PLoS ONE 3(2): e1627.
doi: 10.1371/journal.pone.0001627
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA used to identify rabbit CD8 positive T cells by immunofluorescence
Image caption:
Gating strategy for CD8+ T cells (PBMCs or CD8TpM) after co-culture with BT or EBL cells.
From: Myster F, Gong M-J, Javaux J, Suárez NM, Wilkie GS, Connelley T, et al. (2020)
Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever.
PLoS Pathog 16(3): e1008405.
doi: 10.1371/journal.ppat.1008405
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576F) used to evaluate CD8 expression on cells HSV-1 infected rabbits by flow cytometry.
Image caption:
Differential gene expression in HSV-specific CD8+ T cells from HSV-1 infected symptomatic vs. asymptomatic individuals.
Frequencies of CD8+ T cells specific to HLA-A*0201-restricted HSV-1 gB561–567 and VP11/12702–710 epitopes detected by FACS in SYMP vs. ASYMP individuals.
From: Prakash S, Roy S, Srivastava R, Coulon PG, Dhanushkodi NR, Vahed H, Jankeel A, Geertsema R, Amezquita C, Nguyen L, Messaoudi I, Burkhardt AM, BenMohamed L.
Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes.
Sci Rep. 2020 Aug 14;10(1):13843.
doi: 10.1038/s41598-020-70673-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit CD8 antibody, clone 12.C7, (MCA1576GA) used to label lapine CD8+ T lymphocytes for analysis by flow cytometry.
Image caption:
Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry. PBMC were isolated from K3EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse IgG2a isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or biotinylated rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD- cells.
From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4-
T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42(1):95.
doi: 10.1186/1297-9716-42-95.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit CD8 antibody, clone 12.C7, (MCA1576GA) used to label lapine CD8+ T lymphocytes for analysis by flow cytometry.
Image caption:
CD8+CD4- T cells are the prominent cellular phenotype in lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. (A) Flow cytometry dot plots obtained by multi-colour staining of PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of one representative rabbit of each group at time of euthanasia.
From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4-
T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42(1):95.
doi: 10.1186/1297-9716-42-95.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576F) used to identify rabbit cytotoxic T lymphocytes by flow cytometry as an FMO control.
Image caption:
Representative flow cytometric gating of (A) live Ig+ cells that bind Tp075124–237 PE and APC tetramers but not control 6xHIS PE650 and APC755 tetramers in fractions from PBMC from immunized rabbits or spleen cells from control rabbits (B) depleted or (C) enriched for tetramer-binding B cells using microbeads specific for PE and APC prior to analysis. D. Combined data from three experiments showing the frequency of Tp075124–237 tetramer-binding cells amongst the total Ig+ population for individual rabbits in PBMC seven days after the final immunization, and in the spleen of immunized and control rabbits forty days after T. pallidum challenge. E. Representative flow cytometric gating of IgM− Tp075124–237 tetramer-binding B cells using an FMO control. Of note, cells were stained with antibodies specific for IgM, CD4, and CD8 in the same fluorescent channel but only IgM was left out of the FMO to exclude non-B cells expressing CD8 or CD4 from the population of interest. F. Combined data from three experiments showing the frequency of Tp075124–237 tetramer-binding cells that were IgM− in individual rabbits. G. Combined data from three experiments showing the frequency of IgM− Tp075124–237 tetramer-binding cells amongst the total Ig+ population in individual rabbits. In D, F, and G the lines represent the mean and p values were generated using a two-tailed Student’s t-test.
From: Urselli F, Gomez A, Gray MD, Cameron CE, Taylor JJ.
Identification of antibodies induced by immunization with the syphilis vaccine candidate Tp0751.
Vaccine. 2025 Mar 19;50:126804.
doi: 10.1016/j.vaccine.2025.126804,
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rabbit antibody, clone 12.C7 used to identify cytotoxic T cells from rabbit spleen and blood by flow cytometry.
Image caption:
Example of flow cytometry gating strategy for HSV-1-specific CD8+ T cells in the TG. TG cell suspensions were stained with two distinct panels of antibodies: one for CD8+ T cells and one for CD4+ T cells. The cell population was first plotted on an ungated plot showing size and granularity (A), then a first gate (R1) was performed on lymphocyte area and plotted as single cells (B); then a second gate (R2) was performed and the cell population was re-plotted based on CD8 expression (C) then a third gate (R3) was performed on CD8+ T cells and plotted on tetramer-positive population (D) showing the HSV-specific CD8+ T cells. To study the frequencies of memory CD8+ TCM, CD8+ TEM, and CD8+ TRM cell subsets detected by FACS, we have created a fourth gate (R4) and we plotted the positive populations in different plots such as CD8/CD44, CD8/CD52L, CD8/CD69, CD44/CD69, CD44/CD62L, and CD103/CD69 (E).
From: Chentoufi AA, Prakash S, Vahed H, Karan S, Quadiri A, Nesburn AB, BenMohamed L.
A tissue-targeted prime/pull/keep therapeutic herpes simplex virus vaccine protects against recurrent ocular herpes infection and disease in HLA-A*0201 transgenic rabbits.
J Virol. 2025 May 20;99(5):e0013525.
doi: 10.1128/jvi.00135-25.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F Reset| Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD8 antibody
-
De Smet, W. et al. (1983) Rabbit leukocyte surface antigens defined by monoclonal antibodies.
Eur J Immunol. 13: 919-28. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77: 106-8. -
Schock, A. and Reid, H.W. (1996) Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever.
Vet Microbiol. 53: 111-9. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627. -
Hanson, N.B. & Lanning, D.K. (2008) Microbial induction of B and T cell areas in rabbit appendix.
Dev Comp Immunol. 32 (8): 980-91. -
Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
J Comp Pathol. 138 (2-3): 131-44. -
Pakandl, M. et al. (2008) Dependence of the immune response to coccidiosis on the age of rabbit suckling.
Parasitol Res. 103 (6): 1265-71. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54.
View The Latest Product References
-
Stich N et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
Toxins (Basel). 2 (9): 2272-88. -
Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
J Virol. 85 (14): 6941-54. -
Zhao, L. et al. (2011) Evaluation of immunocompatibility of tissue-engineered periosteum.
Biomed Mater.6:015005. -
Dewals, B.G. & Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42 (1): 95. -
Marques, R.M. et al. (2012) Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection.
Vet Immunol Immunopathol. 150: 181-8. -
Srivastava, R. et al. (2015) A Herpes Simplex Virus Type 1 Human Asymptomatic CD8+ T-Cell Epitopes-Based Vaccine Protects Against Ocular Herpes in a "Humanized" HLA Transgenic Rabbit Model.
Invest Ophthalmol Vis Sci. 56 (6): 4013-28. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Khan AA et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Srivastava, R. et al. (2016) The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
J Virol. 90 (8): 3913-28. -
Khan, A.A. et al. (2018) Human Asymptomatic Epitope Peptide/CXCL10-Based Prime/Pull Vaccine Induces Herpes Simplex Virus-Specific Gamma Interferon-Positive CD107+ CD8+ T Cells That Infiltrate the Corneas and Trigeminal Ganglia of Humanized HLA Transgenic Rabbits and Protect against Ocular Herpes Challenge.
J Virol. 92 (16): e00535-18. -
Gates, K.V. & Griffiths, L.G. (2018) Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.
Immunol Res. 66 (2): 288-98. -
Prakash, S. et al. (2020) Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes.
Sci Rep. 10 (1): 13843. -
Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
Vet Res. 51 (1): 79. -
Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
Pol J Vet Sci. 23 (1): 37-42. -
Myster, F. et al. (2020) Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever.
PLoS Pathog. 16 (3): e1008405. -
Niedźwiedzka-Rystwej, P. et al. (2022) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
Vet Res Commun. 46 (1): 233-42. -
Niedźwiedzka-Rystwej, P. & Deptuła, W. (2023) Crosstalk between Apoptosis and Cytotoxic Lymphocytes (CTLs) in the Course of Lagovirus Europaeus GI.1a Infection in Rabbits.
J Vet Res. 67 (1): 41-47. -
Tansiri, Y. et al. (2021) New potent epitopes from Leptospira borgpetersenii for the stimulation of humoral and cell-mediated immune responses: Experimental and theoretical studies
Informatics in Medicine Unlocked. 25: 100649. -
Urselli, F. et al. (2025) Identification of antibodies induced by immunization with the syphilis vaccine candidate Tp0751.
Vaccine. 50: 126804. -
Chentoufi, A.A. et al. (2025) A tissue-targeted prime/pull/keep therapeutic herpes simplex virus vaccine protects against recurrent ocular herpes infection and disease in HLA-A*0201 transgenic rabbits.
J Virol. 99 (5): e0013525.
MCA1576A647
167150
173663
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD8 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rabbit ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up
