Mouse anti Rabbit CD44 antibody, clone W4/86 recognizes the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly.
Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- RL-5 T cell line glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from an immunized mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD44 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD44 antibody
Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
J Exp Med. 157 (1): 34-46.
Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
Mol Immunol. 21 (1): 95-103.
Galea-Lauri, J. et al. (1993) Characterization of monoclonal antibodies against rabbit CD44: evidence of a role for CD44 in modulating synoviocyte metabolism.
Mol Immunol. 30 (15): 1383-92.
Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42: 95.
Yagi, M. et al. (2010) Hyaluronan modulates proliferation and migration of rabbit fibroblasts derived from flexor tendon epitenon and endotenon.
J Hand Surg Am. 35: 791-6.
Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment
Oncology Letters. May 30 [Epub ahead of print]
Kováč, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
Slovak J Anim Sci., 49,(2): 62–67.
Sugaya, H. et al. (2016) Fate of bone marrow mesenchymal stromal cells following autologous transplantation in a rabbit model of osteonecrosis.
Cytotherapy. 18 (2): 198-204.
Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
<a href="https://jcea.agr.hr/volumes.php?search=Article%253A1883"target="_blank">J Cent Euro Agric. 18 (1): 226-34.</a>
Honda, H. et al. (2017) Hyaluronic Acid Accelerates Tendon-to-Bone Healing After Rotator Cuff Repair.
Am J Sports Med. : 363546517720199.
Kim, H.J. et al. (2019) Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair.
J Control Release. 302: 169-80.
Desando, G. et al. (2018) Short-Term Homing of Hyaluronan-Primed Cells: Therapeutic Implications for Osteoarthritis Treatment.
Tissue Eng Part C Methods. 24 (2): 121-33.
Kulikova, B. et al. (2019) Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
Acta Histochem. 121 (4): 491-9.
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