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CD44 antibody | W4/86

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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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Mouse anti Rabbit CD44 antibody, clone W4/86 recognizes the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly.

Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in western blotting (Blackford 1996).

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
RL-5 T cell line glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from an immunized mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
    Mol Immunol. 21 (1): 95-103.

References for CD44 antibody

  1. Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
    J Exp Med. 157 (1): 34-46.
  2. Galea-Lauri, J. et al. (1993) Characterization of monoclonal antibodies against rabbit CD44: evidence of a role for CD44 in modulating synoviocyte metabolism.
    Mol Immunol. 30 (15): 1383-92.
  3. Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
    Vet Res. 42: 95.
  4. Yagi, M. et al. (2010) Hyaluronan modulates proliferation and migration of rabbit fibroblasts derived from flexor tendon epitenon and endotenon.
    J Hand Surg Am. 35: 791-6.
  5. Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment.
    Oncol Lett. 12 (1): 644-50.
  6. Kováč, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
    Slovak J Anim Sci., 49,(2): 62–67.
  7. Sugaya, H. et al. (2016) Fate of bone marrow mesenchymal stromal cells following autologous transplantation in a rabbit model of osteonecrosis.
    Cytotherapy. 18 (2): 198-204.
  8. Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
    J Cent Euro Agric. 18 (1): 226-34.
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  10. Honda, H. et al. (2017) Hyaluronic Acid Accelerates Tendon-to-Bone Healing After Rotator Cuff Repair.
    Am J Sports Med. 45 (14): 3322-30.
  11. Kim, H.J. et al. (2019) Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair.
    J Control Release. 302: 169-80.
  12. Desando, G. et al. (2018) Short-Term Homing of Hyaluronan-Primed Cells: Therapeutic Implications for Osteoarthritis Treatment.
    Tissue Eng Part C Methods. 24 (2): 121-33.
  13. Kulikova, B. et al. (2019) Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
    Acta Histochem. 121 (4): 491-9.
  14. Kim, D.H. et al. (2019) Rabbit palatum-derived mesenchymal progenitor cells tri-lineage differentiation on 2D substrates and 3D printed constructs.
    J Appl Biomater Funct Mater. 17 (3): 2280800019834520.




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