CD44 antibody | W4/86

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Mouse anti Rabbit CD44

Product Type
Monoclonal Antibody
Clone
W4/86
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA806GA C F IP 0.1 mg
Mouse anti Rabbit CD44 antibody, clone W4/86 recognizes the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly.

Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
RL-5 T cell line glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from an immunized mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD44 antibody

  1. Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
    J Exp Med. 157 (1): 34-46.
  2. Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
    Mol Immunol. 21 (1): 95-103.
  3. Galea-Lauri, J. et al. (1993) Characterization of monoclonal antibodies against rabbit CD44: evidence of a role for CD44 in modulating synoviocyte metabolism.
    Mol Immunol. 30 (15): 1383-92.
  4. Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
    Vet Res. 42: 95.
  5. Yagi, M. et al. (2010) Hyaluronan modulates proliferation and migration of rabbit fibroblasts derived from flexor tendon epitenon and endotenon.
    J Hand Surg Am. 35: 791-6.
  6. Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment
    Oncology Letters. May 30 [Epub ahead of print]
  7. Kováč, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
    Slovak J Anim Sci., 49,(2): 62–67.
  8. Sugaya, H. et al. (2016) Fate of bone marrow mesenchymal stromal cells following autologous transplantation in a rabbit model of osteonecrosis.
    Cytotherapy. 18 (2): 198-204.
  9. Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
    <a href="https://jcea.agr.hr/volumes.php?search=Article%253A1883"target="_blank">J Cent Euro Agric. 18 (1): 226-34.</a>
  10. Honda, H. et al. (2017) Hyaluronic Acid Accelerates Tendon-to-Bone Healing After Rotator Cuff Repair.
    Am J Sports Med. : 363546517720199.