Leishmania Major Surface Protease gp63 antibody | 96/26
Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 (OBT2004) use to evaluate parasite load in canine leukocytes by flow cytometry.
Image caption:
Representative histogram obtained from the CD14+ (FL1) and gp63 (FL2) -labelled flow cytometry analysis on splenic leukocytes from dogs with CanL transfected with miR 21 mimic, negative control (scrambled), and miR 21 inhibitor, all with the presence of Hiperfect (miScript miRNA Mimic and Inhibitor Qiagen, USA) for 67h.
(A) Orange peak population labeled with CD14+ (M11), red peak positivity for gp63 and CD14+ cell (B) in the presence of a miR 21 Mimic (C) in the presence of a negative control (scrambled) (D) and in the presence of the Inhibitor of miR 21 (D).
From: Melo LM, Bragato JP, Venturin GL, Rebech GT, Costa SF, Garcia LE, et al. (2019)
Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes.
PLoS ONE 14(12): e0226192.
doi: 10.1371/journal.pone.0226192
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 recognizes the Leishmania major surface protease (gp63), also known as Leishmanolysin, Promastigote surface endopeptidase or gp63. GP63 is a 477 amino acid ~63 kDa highly conserved protein which is found at high density on many Leishmania species. Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96-126 recognises living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.
Product Details
- Target Species
- Protozoan
- Product Form
- Ascitic Fluid - lyophilised
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preservative Stabilisers
- None present
- Immunogen
- Recombinant baculovirus expressed L. major gp63
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08148
- GO Terms
- GO:0005886 plasma membrane
- GO:0004222 metalloendopeptidase activity
- GO:0007155 cell adhesion
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0031225 anchored to membrane
- Regulatory
- For research purposes only
Applications of Leishmania Major Surface Protease gp63 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Immunoblotting | |||
| Immunofluorescence | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Leishmania Major Surface Protease gp63 antibody
-
Rodríguez, P. et al. (2013) Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania. Genus Parasites
American Journal of Analytical Chemistry. 04 (07): 100-108. -
Melo, L.M. et al. (2019) Induction of miR 21 impairs the anti-Leishmania. response through inhibition of IL-12 in canine splenic leukocytes.
PLoS One. 14 (12): e0226192.
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