Leishmania Major Surface Protease gp63 antibody | 96/26
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From: Melo LM, Bragato JP, Venturin GL, Rebech GT, Costa SF, Garcia LE, et al. (2019) Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes. PLoS ONE 14(12): e0226192. https://doi.org/10.1371/journal.pone.0226192
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From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 recognizes the Leishmania major surface protease (gp63), also known as Leishmanolysin, Promastigote surface endopeptidase or gp63. GP63 is a 477 amino acid ~63 kDa highly conserved protein which is found at high density on many Leishmania species. Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96-126 recognises living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.
Product Details
- Target Species
- Protozoan
- Product Form
- Ascitic Fluid - lyophilised
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preservative Stabilisers
- None present
- Immunogen
- Recombinant baculovirus expressed L. major gp63
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08148
- GO Terms
- GO:0005886 plasma membrane
- GO:0004222 metalloendopeptidase activity
- GO:0007155 cell adhesion
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0031225 anchored to membrane
- Regulatory
- For research purposes only
Applications of Leishmania Major Surface Protease gp63 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Immunoblotting | |||
| Immunofluorescence | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Leishmania Major Surface Protease gp63 antibody
-
Rodríguez, P. et al. (2013) Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania. Genus Parasites
American Journal of Analytical Chemistry. 04 (07): 100-108. -
Melo, L.M. et al. (2019) Induction of miR 21 impairs the anti-Leishmania. response through inhibition of IL-12 in canine splenic leukocytes.
PLoS One. 14 (12): e0226192.
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