Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 recognizes the Leishmania major surface protease (gp63), also known as Leishmanolysin, Promastigote surface endopeptidase or gp63. GP63 is a 477 amino acid ~63 kDa highly conserved protein which is found at high density on many Leishmania species. Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96-126 recognises living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.
- Target Species
- Product Form
- Ascitic Fluid - lyophilised
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preservative Stabilisers
- None present
- Recombinant baculovirus expressed L. major gp63
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- GO Terms
zinc ion binding
anchored to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Leishmania Major Surface Protease gp63 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Specific References
References for Leishmania Major Surface Protease gp63 antibody
Rodríguez, P. et al. (2013) Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania Genus Parasites.
American Journal of Analytical Chemistry, 4: 100-8.