Leishmania Major Surface Protease gp63 antibody | 96/26

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Mouse anti Leishmania Major Surface Protease gp63

Product Type
Monoclonal Antibody
Clone
96/26
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT2004 E IF 0.5 ml
Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 recognizes the Leishmania major surface protease (gp63), also known as Leishmanolysin, Promastigote surface endopeptidase or gp63. GP63 is a 477 amino acid ~63 kDa highly conserved protein which is found at high density on many Leishmania species. Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96-126 recognises living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.

Product Details

Target Species
Protozoan
Product Form
Ascitic Fluid - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Immunogen
Recombinant baculovirus expressed L. major gp63
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08148 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004222 metalloendopeptidase activity
GO:0007155 cell adhesion
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0031225 anchored to membrane
Regulatory
For research purposes only

Applications of Leishmania Major Surface Protease gp63 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunoblotting
Immunofluorescence 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Leishmania Major Surface Protease gp63 antibody

  1. Rodríguez, P. et al. (2013) Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania Genus Parasites.
    American Journal of Analytical Chemistry, 4: 100-8.