Leishmania Major Surface Protease gp63 antibody | 96/26
Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96/26 recognizes the Leishmania major surface protease (gp63), also known as Leishmanolysin, Promastigote surface endopeptidase or gp63. GP63 is a 477 amino acid ~63 kDa highly conserved protein which is found at high density on many Leishmania species. Mouse anti Leishmania Major Surface Protease gp63 antibody, clone 96-126 recognises living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.
- Target Species
- Product Form
- Ascitic Fluid - lyophilised
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preservative Stabilisers
- None present
- Recombinant baculovirus expressed L. major gp63
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- P08148 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0004222 metalloendopeptidase activity
- GO:0007155 cell adhesion
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0031225 anchored to membrane
- For research purposes only
Applications of Leishmania Major Surface Protease gp63 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Leishmania Major Surface Protease gp63 antibody
Rodríguez, P. et al. (2013) Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania Genus Parasites.
American Journal of Analytical Chemistry, 4: 100-8.