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Kinetoplastid Membrane Protein 11 antibody | L-157

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Mouse anti Kinetoplastid Membrane Protein 11

Product Type
Monoclonal Antibody
Clone
L-157
Isotype
IgG1
Specificity
Kinetoplastid Membrane Protein 11

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OBT2003
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Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 recognizes Kinetoplastid Membrane Protein-11 (KMP-11), previously known as the lipophosphoglycan-associated protein (LPGAP), a 92 amino acid ~11 kDa membrane protein. Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 was originally derived against Leishmania donovani lipophosphoglycan, but found to recognize the tightly associated LPGAP. KMP-11 is found in a wide variety of Kinetoplastid parasites including African trypanosomes, Leishmania and Crithidia (Tolson et al. 1994).

Target Species
Protozoan
Product Form
Ascites - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Immunogen
L. donovani lipophosphoglycan semi purified from epimastigotes.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunoblotting 1/1000 1/3000
Immunofluorescence 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Source Reference

  1. Tolson, D.L. et al. (1989) The immunochemical structure and surface arrangement of Leishmania donovani lipophosphoglycan determined using monoclonal antibodies.
    Mol Biochem Parasitol. 35 (2): 109-18.

References for Kinetoplastid Membrane Protein 11 antibody

  1. Tolson, D.L. et al. (1994) The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation.
    Infect Immun. 62 (11): 4893-9.
  2. Pearson, T.W. et al. (2000) The major cell surface glycoprotein procyclin is a receptor for induction of a novel form of cell death in African trypanosomes in vitro.
    Mol Biochem Parasitol. 111 (2): 333-49.
  3. Jardim, A. et al. (2002) Peroxisomal targeting protein 14 (PEX14) from Leishmania donovani. Molecular, biochemical, and immunocytochemical characterization.
    Mol Biochem Parasitol. 124 (1-2): 51-62.
  4. McNeely, T.B. et al. (1990) Characterization of Leishmania donovani variant clones using anti-lipophosphoglycan monoclonal antibodies.
    Glycobiology. 1 (1): 63-9.
  5. Jardim, A. et al. (1991) The Leishmania donovani lipophosphoglycan T lymphocyte-reactive component is a tightly associated protein complex.
    J Immunol. 147 (10): 3538-44.

Synonyms
KMP-11
RRID
AB_619092
UniProt
Q36736
GO Terms
GO:0006952 defense response
GO:0007010 cytoskeleton organization
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0008284 positive regulation of cell proliferation
GO:0042098 T cell proliferation

OBT2003

155687 156151 159246 163278

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