Kinetoplastid Membrane Protein 11 antibody | L-157
Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 recognizes Kinetoplastid Membrane Protein-11 (KMP-11), previously known as the lipophosphoglycan-associated protein (LPGAP), a 92 amino acid ~11 kDa membrane protein. Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 was originally derived against Leishmania donovani lipophosphoglycan, but found to recognize the tightly associated LPGAP. KMP-11 is found in a wide variety of Kinetoplastid parasites including African trypanosomes, Leishmania and Crithidia (Tolson et al. 1994).
- Target Species
- Product Form
- Ascites - lyophilised
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preservative Stabilisers
- None present
- L. donovani lipophosphoglycan semi purified from epimastigotes.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- Q36736 Related reagents
- GO Terms
- GO:0006952 defense response
- GO:0007010 cytoskeleton organization
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0008284 positive regulation of cell proliferation
- GO:0042098 T cell proliferation
- For research purposes only
Applications of Kinetoplastid Membrane Protein 11 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Specific References
Tolson, D.L. et al. (1989) The immunochemical structure and surface arrangement of Leishmania donovani lipophosphoglycan determined using monoclonal antibodies.
Mol Biochem Parasitol. 35 (2): 109-18.
References for Kinetoplastid Membrane Protein 11 antibody
Tolson, D.L. et al. (1994) The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation.
Infect Immun. 62 (11): 4893-9.
Pearson, T.W. et al. (2000) The major cell surface glycoprotein procyclin is a receptor for induction of a novel form of cell death in African trypanosomes in vitro.
Mol Biochem Parasitol. 111 (2): 333-49.
Jardim, A. et al. (2002) Peroxisomal targeting protein 14 (PEX14) from Leishmania donovani. Molecular, biochemical, and immunocytochemical characterization.
Mol Biochem Parasitol. 124 (1-2): 51-62.
McNeely, T.B. et al. (1990) Characterization of Leishmania donovani variant clones using anti-lipophosphoglycan monoclonal antibodies.
Glycobiology. 1 (1): 63-9.
Jardim, A. et al. (1991) The Leishmania donovani lipophosphoglycan T lymphocyte-reactive component is a tightly associated protein complex.
J Immunol. 147 (10): 3538-44.