SWC8 antibody | MIL3

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Mouse anti Pig SWC8

Product Type: Monoclonal Antibody
Clone: MIL3
Isotype: IgM

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1219	C F			2 ml

Mouse anti Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.

Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218GA) to distinguish between monocytes and granulocytes (Haverson et al. 1994).

Product Details

Target Species

Pig

Product Form

Tissue Culture Supernatant - liquid

Preparation

Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Porcine Lamina Propria Leucocytes.

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of SWC8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			1/20
Immunohistology - Frozen		1/10	1/50

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgM:FITC	HCA040F	0.1 mg	F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)	STAR138A	1 ml	C E P WB
Goat anti Mouse IgM:RPE	STAR86PE	0.5 ml	F
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgM Negative Control	MCA692	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for SWC8 antibody

Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45.
Lunney, J.K. (1993) Characterization of swine leukocyte differentiation antigens.
Immunol Today. 14 (4): 147-8.
Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus.
J Gen Virol. 82 (Pt 6): 1309-18.
Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78.
Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology. 110: 440-9.
Barnard, A.L. et al. (2005) Immune response characteristics following emergency vaccination of pigs against foot-and-mouth disease.
Vaccine. 23: 1037-47.
Chen, L. et al. (2003) Macrophages and MHC class II positive dendritiform cells in the iris and choroid of the pig.
Curr Eye Res. 26: 291-6.
Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64.