SWC8 antibody | MIL3

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MCA1219
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F 2 ml loader
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Mouse anti Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.

Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218GA) to distinguish between monocytes and granulocytes (Haverson et al. 1994).

Target Species
Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Porcine Lamina Propria Leucocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

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References for SWC8 antibody

  1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
    J Immunol Methods. 170 (2): 233-45.
  2. Lunney, J.K. (1993) Characterization of swine leukocyte differentiation antigens.
    Immunol Today. 14 (4): 147-8.
  3. Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus.
    J Gen Virol. 82 (Pt 6): 1309-18.
  4. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
    Vet Immunol Immunopathol. 124: 367-78.
  5. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
    Immunology. 110: 440-9.
  6. Barnard, A.L. et al. (2005) Immune response characteristics following emergency vaccination of pigs against foot-and-mouth disease.
    Vaccine. 23: 1037-47.
  7. Chen, L. et al. (2003) Macrophages and MHC class II positive dendritiform cells in the iris and choroid of the pig.
    Curr Eye Res. 26: 291-6.
  8. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
    Vet Res. 41: 64.
  9. View The Latest Product References
  10. LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
    Vaccine. 34 (7): 914-22.
  11. Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

RRID
AB_322076
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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