SWC8 antibody | MIL3
No associated image currently available
Mouse anti Pig SWC8
- Product Type
- Monoclonal Antibody
- Clone
- MIL3
- Isotype
- IgM
- Specificity
- SWC8
Antibody Quality and Performance Guarantee
| Mouse anti Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8. Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218GA) to distinguish between monocytes and granulocytes (Haverson et al. 1994). |
- Target Species
- Pig
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Porcine Lamina Propria Leucocytes.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | ||||||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgM Negative Control | MCA692 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgM Negative Control | ||||||
References for SWC8 antibody
-
Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45. -
Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus.
J Gen Virol. 82 (Pt 6): 1309-18. -
Chen, L. et al. (2003) Macrophages and MHC class II positive dendritiform cells in the iris and choroid of the pig.
Curr Eye Res. 26: 291-6. -
Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology. 110: 440-9. -
Barnard, A.L. et al. (2005) Immune response characteristics following emergency vaccination of pigs against foot-and-mouth disease.
Vaccine. 23: 1037-47. -
Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78. -
Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64. -
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22.
View The Latest Product References
-
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_322076
MCA1219
152844
168715
1703
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Pig ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up