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Monocyte/Granulocyte antibody | 74-22-15

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Mouse anti Pig Monocyte/Granulocyte:RPE

Product Type
Monoclonal Antibody
Clone
74-22-15
Isotype
IgG1
Specificity
Monocyte/Granulocyte

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Product Code Applications Pack Size List Price Your Price Qty
MCA6100PE
Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 thumbnail image 1 Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 thumbnail image 2
Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 gallery image 1
Figure A. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and RPE conjugated Mouse anti Pig Monocyte/Granulocyte (MCA6100PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 gallery image 2
Figure A. FITC conjugated Mouse anti Pig Granulocytes (MCA2600F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Pig Granulocytes (MCA2600F) and RPE conjugated Mouse anti Pig Monocyte/Granulocyte (MCA6100PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15, recognizes 90% of pig monocytes and granulocytes, in addition to 5% of lymphocytes, in peripheral blood. The target of Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15 is CD172a, similarly to clone BL1H7 (Haverson et al. 1994, Blecha et al. 1994). CD172a/SWC3a is expressed on monocytes, dendritic cells, and granulocytes.

Target Species
Pig
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Immunogen
Fresh dd miniature swine thymocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Pig CD14:FITC MCA1218F F 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Pig CD14:FITC

References for Monocyte/Granulocyte antibody

  1. Labarque, G.G. et al. (2000) Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs.
    J Gen Virol. 81 (Pt 5): 1327-34.
  2. Blecha, F. et al. (1994) Workshop studies on monoclonal antibodies reactive against porcine myeloid cells.
    Vet Immunol Immunopathol. 43 (1-3): 269-72.
  3. Jarosz, ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
    Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].
  4. Sedaghat-Rostami, E. et al. (2025) Porcine respiratory coronavirus as a model for acute respiratory disease: mechanisms of different infection outcomes.
    J Immunol. Apr 30 [Epub ahead of print].

Synonyms
CD172a
SWC3a
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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