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IL-18 antibody | 5-C-5

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Mouse anti Pig Interleukin-18:Biotin

Product Type
Monoclonal Antibody
Clone
5-C-5
Isotype
IgG1
Specificity
IL-18

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Product Code Applications Pack Size List Price Your Price Qty
MCA2094B
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E 0.25 mg loader
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Mouse anti Pig Interleukin-18 antibody, clone 5-C-5 recognizes natural and recombinant porcine IL-18. Mouse anti Pig Interleukin-18 antibody, clone 5-C-5 does not recognize porcine IL-1beta, IL-8, IL-12 or IFNgamma, nor does it cross react with human or murine IL-18.

Target Species
Pig
Product Form
Purified IgG conjugated to biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Biotin conjugated Mouse anti Pig interleukin-18 antibody, clone 5-C-5 may be used as a detection reagent in a sandwich ELISA assays in combination with MCA2093 as capture reagent.

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References for IL-18 antibody

  1. Muneta, Y. et al. (2000) Detection of porcine interleukin-18 by sandwich ELISA and immunohistochemical staining using its monoclonal antibodies.
    J Interferon Cytokine Res. 20 (3): 331-6.
  2. Lee, B.M. et al. (2011) Enhanced protection against infection with transmissible gastroenteritis virus in piglets by oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing swine interferon-α and interleukin-18.
    Comp Immunol Microbiol Infect Dis. 34 (4): 369-80.

Synonyms
IGIF
RRID
AB_2123662
UniProt
O19073
Entrez Gene
IL18
GO Terms
GO:0001525 angiogenesis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0008625 induction of apoptosis via death domain receptors
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation

MCA2094B

0712 159968 160973 164561

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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