CD52 antibody | 11/305/44
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|Mouse anti Pig CD52, clone 11/305/44 is a monoclonal antibody recognizing the porcine homologue of human CD52, a ~19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells.
Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). SWC1 is the porcine orthologue to human CD52, expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Porcine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
References for CD52 antibody
Leitner, J. et al. (2012) Porcine SWC1 is CD52--final determination by the use of a retroviral cDNA expression library.
Vet Immunol Immunopathol. 146 (1): 27-34.
Lunney, J.K. et al. (1994) Overview of the First International Workshop to Define Swine Leukocyte Cluster of Differentiation (CD) Antigens.
Vet Immunol Immunopathol. 43: 193-206.
Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35.
Shao, L. et al. (2016) Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs.
Vet Immunol Immunopathol. 171: 7-16.
Piriou-Guzylack, L. et al. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54
Sánchez, C. et al. (1999) The Porcine 2A10 Antigen is Homologous to Human CD163 and Related to Macrophage Differentiation.
J Immunol. 162: 5230-7.
Mccullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology. 98 (2): 203-12.
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