B Cell Subset antibody | BB6-10A10

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Mouse anti Pig B Cell Subset:FITC

Mouse anti Pig B Cell Subset

Mouse anti Pig B Cell Subset:RPE

Product Type
Monoclonal Antibody
Clone
BB6-10A10
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA6099F
Datasheet
F
MSDS
0.1 mg loader

MCA6099GA
Datasheet
F
MSDS
0.1 mg loader

MCA6099PE
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Pig B cell subset antibody, clone BB6-10A10, appears to recognize an immature pig B cell population. Clone BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood, 22-23% in mesenteric lymph nodes, 79-87% in ileal Peyer’s patches, 6-17 in spleen, and 0% in thymus (Boersma et al. 2001).

Product Details

Target Species
Pig
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Product Form
Purified IgM conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgM prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgM prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgM prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
<10% Sucrose
Approx. Protein Concentrations
IgM concentration 0.5 mg/ml
Approx. Protein Concentrations
IgM concentration 0.5 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of B Cell Subset antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Pig CD45RA:RPE MCA1751PE F 100 Tests loader
Mouse anti Pig CD45RA MCA1751GA C F P 0.1 mg loader
Mouse anti Pig CD45RA:FITC MCA1751F F 0.1 mg loader

Product Specific References

References for B Cell Subset antibody

  1. Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
    Vet Immunol Immunopathol. 80 (1-2): 63-78.
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