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Ly-6C antibody | ER-MP20

Rat anti Mouse Ly-6C:StarBright Violet 570

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price

Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.

Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).

Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.

Target Species
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Balb/c macrophage precursor cell hybrids.
Fusion Partners
Spleen cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 404 571
For research purposes only
12 months from date of despatch
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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Mouse Seroblock FcR BUF041B F 0.5 mg loader
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References for Ly-6C antibody

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    Eur J Immunol. 20 (1): 27-34.
  3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
    J Immunol Methods. 217 (1-2): 27-39.
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  5. Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
    J Invest Dermatol. 121: 280-8.
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  9. View The Latest Product References
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    Eur J Immunol. 39 (12): 3538-51.
  24. Bossaller, L. et al. (2016) TLR9 Deficiency Leads to Accelerated Renal Disease and Myeloid Lineage Abnormalities in Pristane-Induced Murine Lupus.
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    J Leukoc Biol. 97 (1): 161-9.
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    J Clin Exp Hematop. 52 (3): 171-7.
  27. Van den Bossche. J. et al. (2012) Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
    Scand J Immunol. 75 (6): 588-98.
  28. Houthuys, E. et al. (2010) A method for the isolation and purification of mouse peripheral blood monocytes.
    J Immunol Methods. 359 (1-2): 1-10.
  29. Greifenberg, V. et al. (2009) Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development.
    Eur J Immunol. 39 (10): 2865-76.
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    Genome Biol. 19 (1): 126.
  37. Pluijmert, N.J. et al. (2020) Effects on cardiac function, remodeling and inflammation following myocardial ischemia-reperfusion injury or unreperfused myocardial infarction in hypercholesterolemic APOE*3-Leiden mice.
    Sci Rep. 10 (1): 16601.
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    J Extracell Vesicles 2022 Jun;11(6):e12228.
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    Glia. 71 (3): 588-601.
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    Sci Rep. 9 (1): 4337.
  44. Heissig, B. et al. (2022) siRNA against CD40 delivered via a fungal recognition receptor ameliorates murine acute graft-versus-host disease.
    EJHaem. 3 (3): 849-61.
  45. Cardona, S.M. et al. (2018) Role of the Fractalkine Receptor in CNS Autoimmune Inflammation: New Approach Utilizing a Mouse Model Expressing the Human CX3CR1(I249/M280) Variant.
    Front Cell Neurosci. 12: 365.
  46. Burzynski, L.C. et al. (2023) Thrombin-activated interleukin-1α drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.
    Cardiovasc Res. 119 (12): 2179-89.

Flow Cytometry


Immunohistology - Frozen

Lymphocyte Antigen 6C2



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