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Kappa Light Chain antibody | OX-20

Rat anti Mouse Kappa Light Chain:HRP

Product Type
Monoclonal Antibody
Kappa Light Chain

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price

Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.

Target Species
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Purified mouse IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunized PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10,000
Western Blotting 1/1000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for Kappa Light Chain antibody

  1. Mason, D.W. et al. (1981) The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies.
    Immunology. 44 (1): 75-87.
  2. White, R.A. et al. (1984) Differentiation markers in the Drosophila ovary.
    J Embryol Exp Morphol. 84: 275-86.
  3. Meyer, K.B. et al. (1990) The importance of the 3'-enhancer region in immunoglobulin kappa gene expression.
    Nucleic Acids Res. 18 (19): 5609-15.
  4. Thomas, M.J. et al. (1999) CD8 T-lymphocyte-mediated regulation of ovalbumin-specific murine IgE responses.
    Int Arch Allergy Immunol. 118 (2-4): 289-91.
  5. Wasserman, G.M. et al. (1993) An approach to development of specific T-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria.
    Infect Immun. 61 (5): 1958-63.
  6. Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
    J Immunol. 168 (1): 216-23.
  7. Salagianni, M. et al. (2007) An essential role for IL-18 in CD8 T cell-mediated suppression of IgE responses.
    J Immunol. 178 (8): 4771-8.
  8. Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
    Mol Ther. 29 (3): 1174-85.
  9. View The Latest Product References

Further Reading

  1. Galfrè, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.
    Nature. 277 (5692): 131-3.

Entrez Gene
GO Terms
GO:0003823 antigen binding


0315 158586 159092 159335 160158

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